Tissue engineering has been recognized as offering an alternative technique to whole-organ and tissue transplantation for diseased, failed, or malfunctioned organs. To reconstruct a new tissue via tissue engineering, the following triad components are needed: (1) cells which are harvested and dissociated from the donor tissue; (2) biomaterials as scaffold substrates in which cells are attached and cultured, resulting in implantation at the desired site of the functioning tissue; and (3) growth factors which promote and/or prevent cell adhesion, proliferation, migration, and differentiation. Of these three key components, scaffolds play a critical role in tissue engineering. This timely book focuses on the preparation and characterization of scaffold biomaterials for the application of tissue-engineered scaffolds. More importantly, it serves as an experimental guidebook on the standardization of the fabrication process and characterization of scaffolding technology.
Protocol for Ice-Particle Leaching Method; Protocol for Salt-Leaching Method; Method and Techniques for Sterilization; Protocol for Decellularized Tissue Scaffold; Protocol for Cytokine Releasing Scaffold; Protocol for Thermally Induced Phase Separation (TIPS) Method; Preparation of Porous Macrobead Scaffold; Protocol for Gel-Pressing Method; Protocol for Centrifugal Method; Protocol for Nano-Thickness Calcium Phosphate Coated Scaffolds; Protocol for Gas Foaming/Salt Leaching Method; Sponge Method for Ceramic Scaffolds; Foaming Method for Ceramic Scaffolds; Cell Culture Using PGA Mesh; Protocol for Wet Spinning Fiber-Bonding Scaffold; Protocol for Nano-Fiber Electrospinning Scaffold; Protocol for Thermo-Sensitive Injectable Gel; Protocol for Freeze Drying Method; Protocol for PLGA Microsphere Scaffold.