This manual provides technical protocols for musculoskeletal research on a translational basis, i.e. a disease-orientated approach. It offers guidance on various laboratory techniques, including cell culture and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal models, CT- and MRI-based densitometry and microarchitectural analysis, biomechanics and functional analysis of orthopedic kinesiology, etc. The content is simple and straightforward, with illustrations and step-by-step procedures as an easy experimental reference for personnel in basic and clinical musculoskeletal research and education. This book will provide a unique multidisciplinary platform for various professions - not only orthopedics, but also biomedical engineering and biomaterial sciences - involving both basic and clinical medicine.
Introduction and Guide for Use (K S Leung); Technique of Bone Molecular Biology and Examples (Bruma); Techniques in Biochemical Investigations (Bruma & Simon); Histological Technique (Francis & Benson); Cell Culture Technique (Louis & Simon); Biomechanics (L Qin); Technique in Assessing Bone Mineral Profile (Vivian & L Qin); Laboratory Animal Models (Edmund & Francis); Microscopic Techniques and Image Analysis (Benson et al.).