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In recent years cellular pathology has become more closely involved in the direct management of patients with the introduction of molecular technologies and targeted therapies. Advanced Techniques in Diagnostic Cellular Pathology introduces students and professionals to these concepts and the key technologies that are influencing clinical practice today. Each chapter is carefully structured to introduce the very latest techniques and describe their clinical purpose, principle, method and application in cellular pathology. The advantages of various methods for preparing, observing and demonstrating cells and tissues employed to assist in diagnosis are explored, in addition to the use of quantitative methods in the detection and diagnosis of disease. Supplementary web-based material including annotated virtual microscope slides is available with the book. This is provided courtesy of i-Path Diagnostics Ltd and can be accessed online from their website www.pathxl.com * Describes the very latest, emerging and established molecular aspects of diagnostic pathology.* A clear, focused approach with each chapter containing a summary, a review of basic principles and clinical applications.*
Includes web-based annotated virtual microscope slides.* Contributions from experienced practitioners contain numerous real-world examples illustrating the use of different diagnostic techniques, and their clinical relevance Written by a team of experienced practitioners this book will prove invaluable both to postgraduate biomedical science students who are training to be cellular pathologists and to professionals working in diagnostic and research laboratories as part of their continuing professional development.
List of Contributors. Preface. List of Tables. List of Figures. Supplementary Materials. 1 Virtual Microscopy (Jim Diamond and David McCleary). 1.1 Introduction. 1.2 Digital (virtual) microscopy: equipment for implementation. 1.3 The virtual slide format. 1.4 Image serving and viewing. 1.5 Applications of virtual microscopy. 1.6 Virtual microscopy in education. 1.7 Computational aspects of virtual microscopy. 1.8 Conclusions. 2 Cytopathology (Mary Hannon-Fletcher). 2.1 Introduction. 2.2 Basic principles. 2.3 Cytodiagnosis. 2.4 Gynaecological cytopathology. 2.5 Conclusions. 3 Flow Cytometry (Ian Dimmick). 3.1 Introduction. 3.2 Sample preparation. 3.3 Principles of the flow cytometer. 3.4 Clinical applications. 3.5 The future for flow cytometry. 3.6 Conclusions. 4 Immunocytochemistry (Perry Maxwell and Merdol Ibrahim). 4.1 Introduction. 4.2 Basic principles. 4.3 Clinical immunocytochemistry. 4.4 Conclusions. 5 Microarray-based Comparative Genomic Hybridization (David S.P. Tan, Rachael Natrajan and Jorge S. Reis-Filho). 5.1 Introduction. 5.2 Principles of array CGH. 5.3 aCGH platforms. 5.4 Choosing the right platform. 5.5 Analysis and validation. 5.6 Finding the target. 5.7 Data integration. 5.8 Clinical applications. 5.9 Conclusions. 6 Tissue In Situ Hybridization (Anthony O' Grady, John O' Loughlin and Hilary Magee). 6.1 Introduction. 6.2 ISH probes. 6.3 Probe labels. 6.4 ISH detection systems. 6.5 Tissue preparation and the ISH procedure. 6.6 Signal amplification. 6.7 ISH controls. 6.8 Clinical applications of tissue ISH. 6.9 ISH automation. 6.10 Image capture and analysis. 6.11 Recent developments and future directions in tissue ISH. 6.12 Conclusions. Index.
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