This book is a contemporary review of selected subjects in liquid chromatography, especially of the technical development, rather than the applications. The subjects are focused in the biomedical and environmental fields. This is also a troubleshooting record. Complex analytical problems such as sensitivity (sensitive detection by chemiluminescence, coulometric detection, laser based detection, necessity of degassing the system for sensitive detection), difficulty (free radical detection by Electron Spin Resonance, Polarimeter for chiral recognition) and reproducibility (packings for chiral separation and stable bonded silica gels) are solved. Theoretically and environmentally important miniaturizations are described. Individual chapters written by specialists provide information beyond what can be found in general textbooks of liquid chromatography.
Advances in liquid chromatography, H Hatano; advance on amino acid analyser, F M Yamamoto & S Ganno; glycoalbumin analyzer, T Uchida; coulometric detector for liquid chromatography, Y Takata; HPLC-ESR detection of free radicals, K Makino & A Moriya; LC-chemiluminescence detection, K Nakajima & K Imai; liquid chromatography and capillary electrophoresis with laser fluorometric detector, T Imasaka; LC-polarimeter, H Nakamura; advantage of on-line degassing, A Nagai; micro-column liquid chromatography, T Takeuti & D Ishi; advantages of micro-column liquid chromatography in hyphenated techniques, K Jinno & C Fujimoto; chiral stationary phases for HPLC, N Oi; synthesized polymer chiral packings, Y Okamoto & E Yashima; protein coated chiral packings and their application, T Miwa & Y Oda; organic polymer packings, Y Kato; designed stable bonded silica gel packings, T Hanai.