Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids addresses several issues related to safe and effective delivery of nucleic acids (NAs) using nanoparticles. A further emphasis would be laid on the mechanism of delivery of NAs, the barriers encountered and the strategies adapted to combat them. An exhaustive account of the advantages as well shortcomings of all the delivery vectors being employed in delivery of various NAs will be provided. On final note the regulatory aspects of nanoparticles mediated NA would be discussed, with focus on their clinical relevance.
The design and development of nucleic acid-based therapeutics for the treatment of diseases arising from genetic abnormalities has made significant progress over the past few years. NAs have been widely explored for the treatment of cancer and infectious diseases or to block cell proliferation and thereby caused diseases. Advances in synthetic oligonucleotide chemistry resulted in synthesis of NAs that are relatively stable in in vivo environments. However, cellular targeting and intracellular delivery of NAs still remains a challenge. Further development of NA-based therapeutics depends on the progress of safe and effective carriers for systemic administration. Nanomedicine has facilitated availability of vectors with diminished cytotoxicity and enhanced efficacy which are rapidly emerging as systems of choice. These vectors protect NAs from enzymatic degradation by forming condensed complexes along with targeted tissue and cellular delivery. During the past few years, a myriad reports have appeared reporting delivery of NAs mediated by nanoparticles. This book will provide an overview of nanoparticles being employed in the in vitro and in vivo delivery of therapeutically relevant NAs like DNA, siRNA, LNA, PNA, etc.
1. Nanomedicine for delivery of therapeutic molecules 2. Nanoparticles as nucleic acid delivery vectors 3. Methods for characterization of nanoparticles 4. Nanoparticles for DNA delivery 5. Nanoparticles for siRNA-mediated gene silencing 6. Nanoparticles for locked nucleic acid delivery 7. Nanoparticles for ribozymes delivery 8. Nanoparticles for DNAzymes delivery 9. Pharmacokinetics and biodistribution of the nanoparticles 10. Nanotoxicology: Evaluation of toxicity potential of nanoparticles 11. Regulatory aspects of nanoparticulate mediated nucleic acid delivery systems 12. Clinical studies and future prospects