Well-versed practitioners detail powerful affinity chromatography methods, ranging from such traditional affinity purification as immunoaffinity chromatography, to the use of state-of-the-art phage-display technology in the discovery of affinity ligands and drugs. Also included are effective and reproducible separations of such small molecules as haptens, protein ligands, and supramolecular structures. Each chapter is devoted to a specific technique and includes an introduction, an explanation of principles, a detailed materials list, and step-by-step instructions that ensure experimental success. Practical notes suggest alternative procedures and describe how to overcome problems that may occur. State-of-the-art and eminently instructive, Affinity Chromatography: Methods and Protocols offers novice and experienced scientists alike a comprehensive collection of proven and readily reproducible techniques that are ideally suited for use not only in drug discovery and development, but also in work on affinity interactions, fermentations, separations, drug targeting, and cell culture.
Introduction: An Overview of Affinity Chromatography Weak Affinity Chromatography Fluidized-Bed Receptor-Affinity Chromatography Site-Specific Immobilization of Antibodies to Protein-G Derivatized Solid Supports Affinity Purification of Monoclonal Antibodies Protein A Mimetic (PAM) Affinity Chromatography: Immunoglobulins Purification Periodate Oxidation of Antibodies for Site-Selective Immobilization in Immunoaffinity Chromatography Mini-Antibody Affinity Chromatography of Lysozyme Nitrocellulose-Based Immunoaffinity Chromatography Immunoaffinity Chromatography Affinity Partitioning of Proteins Boronate Affinity Chromatography Dye-Ligand Affinity Chromatography for Protein Separation and Purification DNA Affinity Chromatography Affinity Chromatography of Pyrogens Western Cross Blot Affinity Perfusion Chromatography Phage Display Technology: Affinity Interactions by Biopanning Phage Display Technology: Identification of Model Peptides for Affinity Chromatography