Amino Acids, Peptides and Proteins in Organic Chemistry: Volume 5 - Analysis and Function of Amino Acids and Peptides (Amino Acids, Peptides and Prote

Amino Acids, Peptides and Proteins in Organic Chemistry: Volume 5 - Analysis and Function of Amino Acids and Peptides (Amino Acids, Peptides and Prote

By: Andrew B. Hughes (series_editor)Hardback

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Description

This is the last of five books in the Amino Acids, Peptides and Proteins in Organic Synthesis series. Closing a gap in the literature, this is the only series to cover this important topic in organic and biochemistry. Drawing upon the combined expertise of the international "who's who" in amino acid research, these volumes represent a real benchmark for amino acid chemistry, providing a comprehensive discussion of the occurrence, uses and applications of amino acids and, by extension, their polymeric forms, peptides and proteins. The practical value of each volume is heightened by the inclusion of experimental procedures. The 5 volumes cover the following topics: Volume 1: Origins and Synthesis of Amino Acids Volume 2: Modified Amino Acids, Organocatalysis and Enzymes Volume 3: Building Blocks, Catalysis and Coupling Chemistry Volume 4: Protection Reactions, Medicinal Chemistry, Combinatorial Synthesis Volume 5: Analysis and Function of Amino Acids and Peptides Volume 5 of this series presents a wealth of methods to analyze amino acids and peptides. Classical approaches are described, such as X-ray analysis, chromatographic methods, NMR, AFM, mass spectrometry and 2D-gel electrophoresis, as well as newer approaches, including Surface Plasmon Resonance and array technologies. Originally planned as a six volume series, Amino Acids, Peptides and Proteins in Organic Chemistry now completes with five volumes but remains comprehensive in both scope and coverage. Further information about the 5 Volume Set and purchasing details can be viewed here.

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About Author

Andrew Hughes is a Reader and Head of the Department of Chemistry, La Trobe University, Melbourne, Australia. He obtained his degrees from the University of Western Australia. Post-doctoral appointments at the University of Cambridge starting 1989 included 3 years working with Professor Andrew Holmes before joining Professor Steven Ley's group in 1993. While at Cambridge he was appointed the Shell Research Fellow at Robinson College.

Contents

List of Contributors XV 1 Mass Spectrometry of Amino Acids and Proteins 1 Simin D. Maleknia and Richard Johnson 1.1 Introduction 1 1.2 Basic Protein Chemistry and How it Relates to MS 21 1.3 Sample Preparation and Data Acquisition 28 1.4 Data Analysis of LC-MS/MS (or CE-MS/MS) of Mixtures 32 1.5 MS of Protein Structure, Folding, and Interactions 36 1.6 Conclusions and Perspectives 40 References 40 2 X-Ray Structure Determination of Proteins and Peptides 51 Andrew J. Fisher 2.1 Introduction 51 2.2 Growing Crystals 55 2.3 Symmetry and Space Groups 62 2.4 X-Ray Scattering and Diffraction 67 2.5 Collecting and Processing Diffraction Data 82 2.6 Solving the Structure (Determining Phases) 83 2.7 Analyzing and Refining the Structure 90 References 94 3 Nuclear Magnetic Resonance of Amino Acids, Peptides, and Proteins 97 Andrea Bernini and Pierandrea Temussi 3.1 Introduction 97 3.2 Amino Acids 101 3.3 Peptides 113 3.4 Proteins 129 3.5 Conclusions 145 References 146 4 Structure and Activity of N-Methylated Peptides 155 Raymond S. Norton 4.1 Introduction 155 4.2 Conformational Effects of N-Methylation 157 4.3 Effects of N-Methylation on Bioactive Peptides 159 4.4 Concluding Remarks 162 References 163 5 High-Performance Liquid Chromatography of Peptides and Proteins 167 Reinhard I. Boysen and Milton T.W. Hearn 5.1 Introduction 167 5.2 Basic Terms and Concepts in Chromatography 169 5.3 Chemical Structure of Peptides and Proteins 173 5.4 HPLC Separation Modes in Peptide and Protein Analysis 177 5.5 Method Development from Analytical to Preparative Scale Illustrated for HP-RPC 189 5.6 Multidimensional HPLC 198 5.7 Conclusions 206 References 207 6 Local Surface Plasmon Resonance and Electrochemical Biosensing Systems for Analyzing Functional Peptides 211 Masato Saito and Eiichi Tamiya 6.1 Localized Surface Plasmon Resonance (LSPR)-Based Microfluidics Biosensor for the Detection of Insulin Peptide Hormone 211 6.2 Electrochemical LSPR-Based Label-Free Detection of Melittin 215 6.3 Label-Free Electrochemical Monitoring of b-Amyloid (Ab) Peptide Aggregation 218 References 221 7 Surface Plasmon Resonance Spectroscopy in the Biosciences 225 Jing Yuan, Yinqiu Wu, and Marie-Isabel Aguilar 7.1 Introduction 225 7.2 SPR-Based Optical Biosensors 225 7.3 Principle of Operation of SPR Biosensors 226 7.4 Description of a SPR Instrument 228 7.5 Application of SPR in Immunosensor Design 230 7.6 Application of SPR in Membrane Interactions 234 7.7 Data Analysis 240 7.8 Conclusions 243 References 244 8 Atomic Force Microscopy of Proteins 249 Adam Mechler 8.1 Foreword 249 8.2 AFM 250 8.3 Bioimaging Highlights 253 8.4 Issues 261 8.5 Force Measurements 269 8.6 Liquid Imaging 269 8.7 Sample Preparation for Bioimaging 272 8.8 Outlook 274 References 275 9 Solvent Interactions with Proteins and Other Macromolecules 277 Satoshi Ohtake, Yoshiko Kita, Kouhei Tsumoto, and Tsutomu Arakawa 9.1 Introduction 277 9.2 Solvent Applications 280 9.3 Solvent Application for Viruses 300 9.4 Solvent Application for DNA 310 9.5 Mechanism 314 9.6 Protein Solvent Interactions in Frozen and Freeze-Dried Systems 342 9.7 Conclusions 348 References 349 10 Role of Cysteine 361 Lalla A. Ba, Torsten Burkholz, Thomas Schneider, and Claus Jacob 10.1 Sulfur: A Redox Chameleon with Many Faces 361 10.2 Three Faces of Thiols: Nucleophilicity, Redox Activity, and Metal Binding 365 10.3 Towards a Dynamic Picture of Disulfide Bonds 371 10.4 Chemical Protection and Regulation via S-Thiolation 374 10.5 Dormant Catalytic Sites 378 10.6 Peroxiredoxin/Sulfiredoxin Catalysis and Control Pathway 379 10.7 Higher Sulfur Oxidation States: From the Shadows to the Heart of Biological Sulfur Chemistry 384 10.8 Cysteine as a Target for Oxidants, Metal Ions, and Drug Molecules 388 10.9 Conclusions and Outlook 390 References 391 11 Role of Disulfide Bonds in Peptide and Protein Conformation 395 Keith K. Khoo and Raymond S. Norton 11.1 Introduction 395 11.2 Probing the Role of Disulfide Bonds 396 11.3 Contribution of Disulfide Bonds to Protein Stability 396 11.4 Role of Disulfide Bonds in Protein Folding 397 11.5 Role of Individual Disulfide Bonds in Protein Structure 399 11.6 Disulfide Bonds in Protein Dynamics 401 11.7 Disulfide Bonding Patterns and Protein Topology 403 11.8 Applications 408 11.9 Conclusions 409 References 410 12 Quantitative Mass Spectrometry-Based Proteomics 419 Shao-En Ong 12.1 Introduction 419 12.2 Quantification in Biological MS 420 12.3 Identifying Proteins Interacting with Small Molecules with Quantitative Proteomics 430 12.4 Conclusions 433 References 434 13 Two-Dimensional Gel Electrophoresis and Protein/Polypeptide Assignment 439 Takashi Manabe and Ya Jin 13.1 Introduction 439 13.2 Aim of Protein Analysis and Development of 2-DE Techniques 439 13.3 Current Status of 2-DE Techniques 441 13.4 Development of Protein Assignment Techniques on 2-DE Gels and Current Status of Mass Spectrometric Techniques 452 13.5 Conclusions 460 References 460 14 Bioinformatics Tools for Detecting Post-Translational Modifications in Mass Spectrometry Data 463 Patricia M. Palagi, Erik Arhne, MarKus Muller, and Frederique Lisacek 14.1 Introduction 463 14.2 PTM Discovery with MS 465 14.3 Database Resources for PTM Analysis 470 14.4 Conclusions 473 References 473 Index 477

Product Details

  • publication date: 19/10/2011
  • ISBN13: 9783527321049
  • Format: Hardback
  • Number Of Pages: 508
  • ID: 9783527321049
  • weight: 1122
  • ISBN10: 3527321047

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