An Introduction to Biotechnology: The Science, Technology and Medical Applications
By: Dr. W. T. Godbey (author)Hardback
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An Introduction to Biotechnology is a biotechnology textbook aimed at undergraduates. It covers the basics of cell biology, biochemistry and molecular biology, and introduces laboratory techniques specific to the technologies addressed in the book; it addresses specific biotechnologies at both the theoretical and application levels.Biotechnology is a field that encompasses both basic science and engineering. There are currently few, if any, biotechnology textbooks that adequately address both areas. Engineering books are equation-heavy and are written in a manner that is very difficult for the non-engineer to understand. Numerous other attempts to present biotechnology are written in a flowery manner with little substance. The author holds one of the first PhDs granted in both biosciences and bioengineering. He is more than an author enamoured with the wow-factor associated with biotechnology; he is a practicing researcher in gene therapy, cell/tissue engineering, and other areas and has been involved with emerging technologies for over a decade. Having made the assertion that there is no acceptable text for teaching a course to introduce biotechnology to both scientists and engineers, the author committed himself to resolving the issue by writing his own.
W. T. Godbey is the Paul H. and Donna D. Assistant Professor in the Department of Chemical and Bimolecular Engineering at Tulane University. He received his B.S. in Mathematics from Southern Methodist University in 1988. After a successful period that involved starting his own software design and development company in Dallas, Texas, he joined the fields of science and engineering and earned his PhD as a National Science Foundation Graduate Fellow from the Institute for Biosciences and Bioengineering at Rice University in 2000. From 2000-2003 he was a postdoctoral fellow at Childrens Hospital, Boston and Harvard Medical School. He joined the Tulane University faculty in 2003.
List of FiguresList of TablesPrefaceAbout the AuthorChapter 1: MembranesAbstract1.1 Membrane Lipids1.2 Cholesterol1.3 Membrane ProteinsQuestionsChapter 2: ProteinsAbstract2.1 Amino Acids2.2 Protein Structure2.3 The Hydrophobic Effect2.4 A Return to MembranesQuestionsChapter 3: Cellular TransportAbstract3.1 Membrane Transporters3.2 Vesicular Transporters: Endocytosis3.3 Receptor Fates3.4 Lysosomes Are for Degradation, But Are They Safe?QuestionsChapter 4: Genes: The Blueprints for ProteinsAbstract4.1 Nucleotides and Nucleic Acids4.2 From Genes to ProteinsQuestionsChapter 5: Cell GrowthAbstract5.1 The Eukaryotic Cell Cycle5.2 Growth Curves and Their Phases5.3 Mathematics of the Growth Curve5.4 Counting Cell Numbers5.5 Counting Cell Mass5.6 Scale-UpQuestionsChapter 6: Microbial KillingAbstract6.1 The Gram Stain6.2 Microbial Resistance to Killing6.3 Sterilization, Disinfection, and Sanitization6.4 Microbial Cell DeathQuestionsChapter 7: Cell Culture and the Eukaryotic Cells Used in BiotechnologyAbstract7.1 Adherent Cells Versus Nonadherent Cells7.2 Primary Cells, Cancer Cells, and Cell LinesQuestionsChapter 8: FluorescenceAbstract8.1 Stokes' Experiments8.2 Fluorophore Properties8.3 Fluorescence Detection8.4 FRETQuestionsChapter 9: Locating Transcriptional Control Regions: Deletion AnalysisAbstract9.1 An Example of Deletion AnalysisQuestionsChapter 10: Agarose GelsAbstract10.1 Application of Agarose Gels: Gel Shift10.2 Application of Agarose Gels: DNA Footprinting10.3 Application of Agarose Gels: Restriction AnalysisQuestionsChapter 11: The Polymerase Chain ReactionAbstract11.1 Melt11.2 Anneal11.3 Extend11.4 PCR Loops11.5 An Application of Traditional PCR11.6 Traditional Versus Real-Time PCR11.7 Real-Time PCRQuestionsChapter 12: Genetic EngineeringAbstract12.1 Plasmid Architecture12.2 Molecular Cloning12.3 A Single Plasmid Is Not Enough12.4 Spectrophotometry12.5 What We Have Learned so FarQuestionsChapter 13: Gene DeliveryAbstract13.1 Gene Delivery Vehicles: An Overview13.2 Gene Methods in Greater Detail13.3 Preparation of Nonviral Gene Delivery ComplexesQuestionsChapter 14: RNAiAbstract14.1 Cosuppression14.2 RNA Interference14.3 miRNAQuestionsChapter 15: DNA FingerprintingAbstract15.1 Older DNA Fingerprinting Uses RFLPs15.2 Newer DNA Fingerprinting Uses STRsQuestionsChapter 16: Fermentation, Beer, and BiofuelsAbstract16.1 Glycolysis16.2 Fermentation16.3 The Production of Beer16.4 Fermentation to Produce BiofuelsQuestionsChapter 17: Stem Cells and Tissue EngineeringAbstract17.1 Potential17.2 An Alternate View of Stem Cells17.3 Using Stem Cells17.4 Tissue Engineering and Regenerative Medicine17.5 Bioreactors17.6 Polymeric Scaffolds17.7 Bringing it all Together: A Tissue Engineering ApplicationQuestionsChapter 18: TransgenicsAbstract18.1 Ice-Minus Bacteria18.2 Bt Plants18.3 Herbicide Resistance18.4 Tomatoes18.5 Rice18.6 Terminators and TraitorsQuestionsChapter 19: Patents and LicensesAbstract19.1 Types of Patents19.2 Licenses19.3 After a License Is GrantedQuestionsIndex
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