An Introduction to Biotechnology is a biotechnology textbook aimed at undergraduates. It covers the basics of cell biology, biochemistry and molecular biology, and introduces laboratory techniques specific to the technologies addressed in the book; it addresses specific biotechnologies at both the theoretical and application levels.
Biotechnology is a field that encompasses both basic science and engineering. There are currently few, if any, biotechnology textbooks that adequately address both areas. Engineering books are equation-heavy and are written in a manner that is very difficult for the non-engineer to understand. Numerous other attempts to present biotechnology are written in a flowery manner with little substance. The author holds one of the first PhDs granted in both biosciences and bioengineering. He is more than an author enamoured with the wow-factor associated with biotechnology; he is a practicing researcher in gene therapy, cell/tissue engineering, and other areas and has been involved with emerging technologies for over a decade. Having made the assertion that there is no acceptable text for teaching a course to introduce biotechnology to both scientists and engineers, the author committed himself to resolving the issue by writing his own.
W. T. Godbey is the Paul H. and Donna D. Assistant Professor in the Department of Chemical and Bimolecular Engineering at Tulane University. He received his B.S. in Mathematics from Southern Methodist University in 1988. After a successful period that involved starting his own software design and development company in Dallas, Texas, he joined the fields of science and engineering and earned his PhD as a National Science Foundation Graduate Fellow from the Institute for Biosciences and Bioengineering at Rice University in 2000. From 2000-2003 he was a postdoctoral fellow at Childrens Hospital, Boston and Harvard Medical School. He joined the Tulane University faculty in 2003.
List of Figures List of Tables Preface About the Author Chapter 1: Membranes Abstract 1.1 Membrane Lipids 1.2 Cholesterol 1.3 Membrane Proteins Questions Chapter 2: Proteins Abstract 2.1 Amino Acids 2.2 Protein Structure 2.3 The Hydrophobic Effect 2.4 A Return to Membranes Questions Chapter 3: Cellular Transport Abstract 3.1 Membrane Transporters 3.2 Vesicular Transporters: Endocytosis 3.3 Receptor Fates 3.4 Lysosomes Are for Degradation, But Are They Safe? Questions Chapter 4: Genes: The Blueprints for Proteins Abstract 4.1 Nucleotides and Nucleic Acids 4.2 From Genes to Proteins Questions Chapter 5: Cell Growth Abstract 5.1 The Eukaryotic Cell Cycle 5.2 Growth Curves and Their Phases 5.3 Mathematics of the Growth Curve 5.4 Counting Cell Numbers 5.5 Counting Cell Mass 5.6 Scale-Up Questions Chapter 6: Microbial Killing Abstract 6.1 The Gram Stain 6.2 Microbial Resistance to Killing 6.3 Sterilization, Disinfection, and Sanitization 6.4 Microbial Cell Death Questions Chapter 7: Cell Culture and the Eukaryotic Cells Used in Biotechnology Abstract 7.1 Adherent Cells Versus Nonadherent Cells 7.2 Primary Cells, Cancer Cells, and Cell Lines Questions Chapter 8: Fluorescence Abstract 8.1 Stokes' Experiments 8.2 Fluorophore Properties 8.3 Fluorescence Detection 8.4 FRET Questions Chapter 9: Locating Transcriptional Control Regions: Deletion Analysis Abstract 9.1 An Example of Deletion Analysis Questions Chapter 10: Agarose Gels Abstract 10.1 Application of Agarose Gels: Gel Shift 10.2 Application of Agarose Gels: DNA Footprinting 10.3 Application of Agarose Gels: Restriction Analysis Questions Chapter 11: The Polymerase Chain Reaction Abstract 11.1 Melt 11.2 Anneal 11.3 Extend 11.4 PCR Loops 11.5 An Application of Traditional PCR 11.6 Traditional Versus Real-Time PCR 11.7 Real-Time PCR Questions Chapter 12: Genetic Engineering Abstract 12.1 Plasmid Architecture 12.2 Molecular Cloning 12.3 A Single Plasmid Is Not Enough 12.4 Spectrophotometry 12.5 What We Have Learned so Far Questions Chapter 13: Gene Delivery Abstract 13.1 Gene Delivery Vehicles: An Overview 13.2 Gene Methods in Greater Detail 13.3 Preparation of Nonviral Gene Delivery Complexes Questions Chapter 14: RNAi Abstract 14.1 Cosuppression 14.2 RNA Interference 14.3 miRNA Questions Chapter 15: DNA Fingerprinting Abstract 15.1 Older DNA Fingerprinting Uses RFLPs 15.2 Newer DNA Fingerprinting Uses STRs Questions Chapter 16: Fermentation, Beer, and Biofuels Abstract 16.1 Glycolysis 16.2 Fermentation 16.3 The Production of Beer 16.4 Fermentation to Produce Biofuels Questions Chapter 17: Stem Cells and Tissue Engineering Abstract 17.1 Potential 17.2 An Alternate View of Stem Cells 17.3 Using Stem Cells 17.4 Tissue Engineering and Regenerative Medicine 17.5 Bioreactors 17.6 Polymeric Scaffolds 17.7 Bringing it all Together: A Tissue Engineering Application Questions Chapter 18: Transgenics Abstract 18.1 Ice-Minus Bacteria 18.2 Bt Plants 18.3 Herbicide Resistance 18.4 Tomatoes 18.5 Rice 18.6 Terminators and Traitors Questions Chapter 19: Patents and Licenses Abstract 19.1 Types of Patents 19.2 Licenses 19.3 After a License Is Granted Questions Index