Analytical Chemistry of Bacillus Thuringiensis: Symposium : 198th National Meeting : Papers (ACS Symposium Series No 432)
By: Leslie A. Hickle (editor), William L. Fitch (editor)Hardback
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This volume addresses the analytical chemistry involved in identifying and quantifying the toxin proteins present in various varieties of Bacillus thuringiensis (Bt). It concentrates on describing and utilizing analytical techniques for characterizing the insect-active inclusion proteins and exotoxins produced by Bt, including bioassay, DNA probes, immunoassay, polyacrylamide gel electrophoresis, in vitro assays, HPLC, and multi-parameter light scattering technology. It brings together an interdisciplinary group of scientists to discuss multiple approaches to a complex problem. Useful for both the layman and professional, this volume serves as a central reference for the major analytical techniques used in characterizing Bt toxins.
The Analytical Chemistry of Bacillus thuringiensis: An Overview ; Historical Aspects of the Quantification of the Active Ingredient Percentage of Bacillus thuringiensis Products ; Bioassay Methods for Quantification of Bacillus thuringiensis *d-Endotoxin Action ; Identification of Entomocidal Toxins of Bacillus thuringiensis by HPLC ; Characterization of Parasporal Crystal Toxins of Bacillus thuringiensis subspecies kurstaki Strains HD-1 and NRD-12 at the DNA and Protein Levels: Use of Oliogonucleotide Probes and Cyanogen Bromide Mapping ; Development of an HPLC Assay for Bacillus thuringiensis var. san diego *d-Endotoxin ; Use of SDS-Page to Quantify Bacillus thuringiensis *d-Endotoxins ; Quantitative Immunoassay of Insecticidal Proteins in Bacillus thuringiensis Products ; The Light Scattering Characterization of *d-Endotoxin Production in Inclusion Bodies ; Quantification of Bacillus thuringiensis Insect Control Protein as Expressed in Transgenic Plants ; HPLC Analysis of Two *b-Exotoxins Produced by Some Bacillus thuringiensis Strains
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- ID: 9780841218154
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