This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.
Dr John Davis: Visiting Lecturer in Biotechnology, University of Hertfordshire, previously Senior Project Scientist at Bio Products Laboratory, UK.
Contributors. Preface. Abbreviations. Protocols. 1 The Cell Culture Laboratory (Sue Clarke and Janette Dillon). 2 Sterilization (Peter L. Roberts). 3 Microscopy of Living Cells (Colin Gray and Daniel Zicha). 4 Basic Techniques and Media, the Maintenance of Cell Lines, and Safety (John M. Davis). 5 Development and Optimization of Serum- and Protein-free Culture Media (Stephen F. Gorfien and David W. Jayme). 6 Cryopreservation and Banking of Cell Lines (Glyn N. Stacey, Ross Hawkins and Roland A. Fleck). 7 Primary Culture of Specific Cell Types and the Establishment of Cell Lines (Kee Woei Ng, Mohan Chothirakottu Abraham, David Tai Wei Leong, Chris Morris and Jan-Thorsten Schantz). 8 Cloning (John Clarke, Alison Porter and John M. Davis). 9 The Quality Control of Animal Cell Lines and the Prevention, Detection and Cure of Contamination (Peter Thraves and Cathy Rowe). 10 Systems for Cell Culture Scale-up (Jennifer Halsall and John M. Davis). 11 Good Laboratory Practice in the Cell Culture Laboratory (Barbara Orton). Index. Colour plates.