Animal Cell Technology: from Biopharmaceuticals to Gene Therapy provides a comprehensive insight into biological and engineering concepts related to mammalian and insect cell technology, as well as an overview of the applications of animal cell technology. Part 1 of the book covers the Fundamentals upon which this technology is based and covers the science underpinning the technology. Part 2 covers the Applications from the production of therapeutic proteins to gene therapy. The authors of the chapters are internationally-recognized in the field of animal cell culture research and have extensive experience in the areas covered in their respective chapters.
Leda R. Castilho - Cell Culture Engineering Laboratory, Chemical Engineering Program - CPPE, Federal University of Rio do Janeiro, Brazil Angela Maria Moraes - School of Chemical Engineering, Department of Biotechnological Processes, State University of Campinas, Brazil Elisabeth F. P. Augusto - Institute of Technological Research of the State of Sao Paulo, Brazil Michael Butler - Department of Microbiology, University of Manitoba, Canada
1. Introduction to Animal Cell Technology 2. Animal Cells: Basic Concepts 3. Cloning and Expression of Heterologous Proteins in Animal Cells 4. Cell Metabolism and its Control in Culture 5. Culture Media for Animal Cells 6. Post-Translational Modification of Recombinant Proteins 7. Mechanisms of Cell Proliferation and Cell Death in Animal Cell Culture In Vitro 8. Mathematical Models for Growth and Product Synthesis in Animal Cell Culture 9. Bioreactors for Animal Cells 10. Monitoring and Control of Cell Cultures 11. Animal Cell Separation 12. Product Purification Processes 13. Quality Control of Biotechnological Products 14. Regulatory Aspects 15. Intellectual Property 16. Recombinant Therapeutic Proteins 17. Monoclonal Antibodies 18. Viral Vaccines: Concepts, Principles and Bioprocesses 19. Bioinsecticides 20. Cell Therapies and Stem Cells 21. Gene Therapy