A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction.
Chapter coverage includes: * Stem cell analysis and sorting using side population * Flow cytometry in the study of proliferation and apoptosis * Stem cell biology and application * Identification and isolation of very small embryonic-like stem cells from murine and human specimens * Hematopoietic stem cells-issues in enumeration * Human embryonic stem cells: long-term culture and cardiovascular differentiation * Limbal stem cells and corneal regeneration * Flow cytometric sorting of spermatogonial stem cells * Breast cancer stem cells * Stem cell marker expression in cells from body cavity fluids This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.
CONTRIBUTORS. PREFACE. 1 Basics of Flow Cytometry (H. Krishnamurthy and L. Scott Cram). 2 Practical Considerations for Flow Cytometric Sorting of Stem Cells (Geoffrey W. Osborne). 3 Stem Cell Analysis and Sorting Using Side Population Analysis (William Telford). 4 Flow Cytometry in the Study of Proliferation and Apoptosis (Michael G. Ormerod and Ronald M. Hamelik). 5 Flow Cytometric Analysis of Drug Transport and Efflux in Stem Cells (Awtar Krishan and Ronald M. Hamelik). 6 Stem Cell Biology and Application (Swapnil Totey, Rajarshi Pal, Murali Krishna Mamidi, Vijayendran Govindasamy, and Satish Totey). 7 Identification and Isolation of Very Small Embryonic-like Stem Cells from Murine and Human Specimens (Ewa K. Zuba-Surma, Dong-Myung Shin, Izabella Klich, Janina Ratajczak, Magda Kucia, and Mariusz Z. Ratajczak). 8 Electronic Volume of Hematopoietic Stem Cells (Siddharth Sharma and Awtar Krishan0. 9 Hematopoietic Stem Cells: Issues in Enumeration (Michael Keeney and D. Robert Sutherland). 10 Embryonic Stem Cells: Development and Characterization (Vijay Bhaskar R. Konala, Villoo Morawala-Patell, and Aparna Khanna). 11 Human Embryonic Stem Cells: Long-Term Culture and Cardiovascular Differentiation (Maneesha Inamdar). 12 Mesenchymal Stromal Cells and Their Clinical Applications (Jyoti Kode and Vivek Tanavde). 13 Circulating Adult Stem Cells of Hematopoietic Origin for Vascular and Neural Regeneration (Lissy K. Krishnan). 14 Flow Cytometric Characterization of Neural Progenitors Derived from Human Pluripotent Stem Cells (Raj R. Rao, Sujoy K. Dhara, Shilpa Iyer, and David W. Machacek). 15 Limbal Stem Cells and Corneal Regeneration (Geeta K. Vemuganti, Murali Mohan Sagar Balla, and Shubha Tiwari). 16 Flow Cytometric Sorting of Spermatogonial Stem Cells (B. S. Srinag, J. M. Kalappurakkal, G.H. Mohan, and H. Krishnamurthy). 17 Breast Cancer Stem Cells (Devaveena Dey and Annapoorni Rangarajan). 18 Tumor Stem Cell Marker Expression in Cells from Body Cavity Fluids (Awtar Krishan, Deepti Sharma, and Ronald M. Hamelik). INDEX.
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