Developmental Hematopoiesis: Methods and Protocols

Developmental Hematopoiesis: Methods and Protocols

By: Margaret H. Baron (author), Margaret H. Baron (editor)Hardback

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This book provides an outstanding collection of protocols for studying hematopoietic development that will be of use to students and researchers. Section 1 covers genetic approaches to hematopoietic development. Section 2 covers two transplantation systems that can be used to follow the determination of cell fate following introduction of embryonic or fetal hematopoietic stem/progenitor cells into mice. Section 3 covers the model systems most widely used for investigating the formation of hematopoietic cells and tissues. Bioinformatics and functional genomics approaches for studying stem cells and their supporting stromal cells.


Part I. Genetic Approaches; Gene Targeting and Transgenic Strategies for Analysis of Hematopoietic Development in the Mouse; Hanna K.A. Mikkola and Stuart H. Orkin; Inducible Transgene Expression in Mouse ES Cells; David T. Ting, Michael Kyba, and George Q. Daley; Quantitative Trait Analysis in the Investigation of Function and Aging in Hematopoietic Stem Cells; Hans-Willem Snoeck; Part II. Analysis of Cell Fate Outcomes in Transplantation Models; Flow Cytometric Analysis of Hematopoietic Development; Edward F. Srour and Mervin C. Yoder; Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells; Elisabeth H. Javazon, Aziz M. Merchant, Enrico Danzer, and Alan W. Flake; Reconstitution of Hematopoiesis Following Transplantation into Neonatal Mice; Scott A. Johnson and Mervin C. Yoder; Part III. Animal Models; Drosophila; Hemocyte Development during Drosophila Embryogenesis; Robert A. Schulz and Nancy Fossett; Xenopus; Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos; Maggie Walmsley, Aldo Ciau-Uitz, and Roger Patient; Fate Mapping Hematopoietic Lineages in the Xenopus Embryo; Mary Constance Lane and Michael D. Sheets; Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J strain); Yumi Izutsu and Mitsugu Maeno; Use of flow cytometry and combined DNA/Surface staining for analysis of hematopoietic development in the Xenopus embryo; James B. Turpen; Zebrafish; Analysis of Hematopoietic Development in the Zebrafish Embryo; Noelle N. Paffett-Lugassy and Leonard I. Zon; Imaging Early Macrophage Differentiation, Migration and Behaviours in Live Zebrafish Embryos; Philippe Herbomel and Jean-Pierre Levraud; Chick; In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune; Systems of the Avian and Mammalian Embryo; Francoise Dieterlen-Lievre, Sophie Creuzet, and Josselyne Salaun; Mouse; Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Development; Margaret H. Baron and Deanna Mohn; Hematopoietic Stem Cell Enrichment from the AGM Region of the Mouse Embryo; Catherine Robin and Elaine Dzierzak; Hematopoietic Stem Cell Development during Mouse Embryogenesis; Julien Y. Bertrand, Sebastien Giroux, Ana Cumano, and Isabelle Godin; Analysis of Hematopoietic Progenitors in the Mouse Embryo; James Palis and Anne Koniski; In Vivo and In Vitro Assays of Thymic Organogenesis; Julie Gordon, Valerie A. Wilson, Billie A. Moore-Scott, Nancy R. Manley, and C. Clare Blackburn; Retroviral Transduction in Fetal Thymic Organ Culture; Bronwyn M. Owens, Robert G. Hawley, and Lisa M. Spain; Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors; Helmut Dolznig, Andrea Kolbus, Uwe Schmidt, Cornelia Strobl, Eva-Maria Deiner, Ernst W. Mullner, and Hartmut Beug; A New Clonal Assay System for Lymphoid and Myeloid Lineages; Hiroshi Kawamoto and Yoshimoto Katsura; Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culture; Kyunghee Choi, Yun Shin Chung, and Weh Jie Zhang; Analysis of the Endothelial Potential of Hematopoietic Stem Cells; Steven M. Guthrie, Sergio Caballero, Robert N. Mames, Maria B. Grant, and Edward W. Scott; Dynamic In Vivo Imaging of Mammalian Hemato-Vascular Development Using Whole Embryo Culture; Elizabeth A. V. Jones, Margaret H. Baron, Scott E. Fraser, and Mary E. Dickinson; Fluorescent protein-cell labeling and its application in time-lapse analysis of hematopoietic differentiation; Matthias Stadtfeld, Florencio Varas, and Thomas Graf; Human

Product Details

  • ISBN13: 9781592598267
  • Format: Hardback
  • Number Of Pages: 550
  • ID: 9781592598267
  • ISBN10: 1592598269

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