Now in full color for a more intuitive learning experience, this new edition of the long-selling reference also features a number of new developments in methodology and the application of enzyme kinetics. Starting with a description of ligand binding equilibria, the experienced author goes on to discuss simple and complex enzyme reactions in kinetic terms. Special cases such as membrane-bound and immobilized enzymes are considered, as is the influence of external conditions, such as temperature and pH value. The final part of the book then covers a range of widely used measurement methods and compares their performance and scope of application. With its unique mix of theory and practical advice, this is an invaluable aid for teaching as well as for experimental work.
Hans Bisswanger was Professor at the Interfaculty Institute of Biochemistry at the University of Tubingen (Germany), where he has developed and taught for many years an intensive course on enzyme kinetics, enzyme technology and ligand binding. His scientific interest lies with structural and regulatory mechanisms of multi-enzyme complexes, thermophilic enzymes and the technical application of immobilized enzymes. He is the author of two well-known books on enzymology that have appeared in different languages and editions.
PART I: Ligand Binding Diffusion Interaction between macromolecules and ligands Identical independent binding sites Non-identical independent binding sites Identical interacting binding sites, cooperativity Non-identical interacting binding sites PART II: Enzyme Kinetics Reaction Order Steady-state kinetics and the Michaelis-Menten Equation Reversible enzyme reactions Enzyme inhibition Multi-substrate reactions Rate equations of complex enzyme mechanisms Allosteric enzymes pH and temperature dependence Isotope exchange kinetics and isotope effects Ribozymes Enyzmes with polymeric substrates Immobilized enzymes Kinetics of transport processes Enzyme reactions at membrane interfaces PART III: Methods Methods for investigating multiple equilibria Electrochemical methods Calorimetry Spectroscopic methods Measurement of fast reactions