Equilibria and Kinetics of Biological Macromolecules provides readers with an understanding of the biophysics of macromolecules. Presented with a pedagogical approach, beginning with introductory concepts, the book addresses thermodynamics, statistical mechanics, ligand binding of proteins, and conformational stability, before focusing on focuses on kinetics and equilibria via discussion of discussion of theory, protein folding, and stochastic models. Placing a strong emphasis on molecular interactions, this reference also contains extensive appendices offer refreshers on probability, deriving grand partial functions, computational methods, helix-coil transition theory, laplace transformation, Poisson equation, defining molecular boundaries, and various equations, among other useful facts.
JAN HERMANS, PhD, is Emeritus Professor in the Department of Biochemistry and Biophysics at the University of North Carolina at Chapel Hill. He is the author of over 130 papers in the field of protein and macromolecular biophysics. BARRY LENTZ, PhD, is Professor in the Department of Biochemistry and Biophysics at the University of North Carolina at Chapel Hill and Director of the UNC Molecular & Cellular Biophysics Program. He has authored roughly 130 original research publications in the field of biophysics, focusing on biomembrane microstructure and cell function.
Preface xix Acknowledgments xxi PART 1 BASIC PRINCIPLES 1 1. Thermodynamics 3 2. Four Basic Quantum Mechanical Models of Nuclear and Electronic Motion: A Synopsis 35 3. Molecular Structure and Interactions 51 4. Water and the Hydrophobic Effect 77 PART 2 STATISTICAL MECHANICS: THE MOLECULAR BASIS OF THERMODYNAMICS 91 5. The Molecular Partition Function 93 6. System Ensembles and Partition Functions 111 7. Sampling Molecular Systems with Simulations 137 PART 3 BINDING TO MACROMOLECULES 161 8. Binding Equilibria 163 9. Thermodynamics of Molecular Interactions 185 10. Elements of Statistical Mechanics of Liquids and Solutions 197 11. Analysis of Binding Equilibria in Terms of Partition Functions 213 12. Coupled Equilibria 223 13. Allosteric Function 239 14. Charged Groups: Binding of Hydrogen Ions, Solvation, and Charge Charge Interactions 255 PART 4 CONFORMATIONAL STABILITY AND CONFORMATION CHANGE 277 15. Some Elements of Polymer Physics 279 16. Helix-Coil Equilibria 291 17. Protein Unfolding Equilibria 311 18. Elasticity of Biological Materials 347 PART 5 KINETICS AND IRREVERSIBLE PROCESSES 357 19. Kinetics 359 20. Kinetics of Protein Folding 389 21. Irreversible and Stochastic Processes 415 APPENDICES 437 Index 491