Presents the proceedings of a conference cosponsored by the Colorado Advanced Technology Institute and the National Institute of Standards and Technology. Reviews recent advances that are expanding the envelope of bioprocessing technology, whether by technology pull or by market push. 32 chapters, each prepared by a conference speaker, address recent notable advances in six major areas: protein and enzyme engineering, biosensors and in situ measurement systems, cell-culture systems, bioreactor engineering and control, bioseparations scaleup, and emerging technologies in bioseparations. A coherent interdisciplinary update covering a far broader range of topics than any previous volume on bioprocessing.
Frontiers in Bioprocessing: An Assessment ; Protein and Enzyme Engineering: The Role of Protein Crystals in Biotechnology and Industry ; Center for Advanced Research in Biotechnology: A Multidisciplinary Approach to Protein Engineering ; Cell-Free Synthesis Bioreactor ; Recombination in Replicating RNAs ; Enantioselective Enzymatic Synthesis of Prostaglandin-Synthons in Multiphase Reaction Media ; Design and Synthesis of a Peptide Having Chymotrypsin-Like Catalytic Activity ; Biosensors and In Situ Measurement Systems: Fiber-Optic Sensors Using Raman and Surface-Enhanced Raman Spectroscopy ; Non-Invasive Spectroscopic Monitoring of a Bioprocess ; In Situ On-Line Optical Fiber Sensor for Fluorescence Monitoring in Bioreactors ; Fiber-Optic Biosensors Incorporating Sustained Release of Reagents ; Whole Cell Biosensors: A Brief Overview and Presentation of a Novel Neuron-Based Approach ; Investigation of Immobilized-Cell Energetics and Metabolism Using NMR Spectroscopy ; Cell-Culture Systems: The Formation of *b-Lactamase Inclusion Bodies in Escherichia coli and Comparison with In Vitro Studies ; Secretion of Heterologous Proteins and Peptides by Pichia pastoris ; Streptomyces Bioprocessing: From Secondary Metabolites to Heterologous Proteins ; Strategy for the Production and Stabilization of Lignin Peroxidase from Phanerochaete chrysosporium in Air-Lift and Stirred Tank Bioreactors ; Bioprocessing of Higher Plant-Cell Cultures ; The Oligosaccharides of Glycoproteins: Factors Affecting Their Synthesis and Their Influence on Glycoprotein Properties ; Modeling the Regulation of Antibody Synthesis and Assembly in a Murine Hybridoma ; Bioreactor Engineering and Control: Modeling of Immobilized Microbial Cells: Influence of Substrate or Product Inhibition on Activity and Biomass Spatial Profiles ; Micromixing in Fermentation-The Response of Microorganisms to Hydrodynamic Forces ; Gas-Liquid Oxygen Transfer in Perfluorochemical-in-Water Dispersions ; Strategies to Minimize Fouling in the Membrane Processing of Biofluids ; Bioseparation Scale-up: Role of Functionalized Membrane Separation Technology in Downstream Processing of Biotechnology-Derived Proteins ; Extraction of Lipases in Aqueous Two-Phase Systems: The Strategy for an Optimization ; Salt-Polyethylene Glycol Two-Phase Aqueous Systems to Purify Proteins and Nucleic Acid Mixtures ; A Polyethylene Glycol-Sodium Chloride Multiphase System for Extraction of Acid Hydrolysates ; Emerging Technologies in Bioseparations: An Expert System for Selection and Synthesis of Protein Purification Processes ; Thermodynamics of Aqueous Mixtures of Salts and Polymers ; Membrane Affinity Technology for Biomolecule Purification and Diagnostics ; Recycling Free-Flow Focusing: Apparatus and Applications