Genomics Protocols (Methods in Molecular Biology v. 175)

Genomics Protocols (Methods in Molecular Biology v. 175)

By: Ramnath Elaswarapu (editor), Michael P. Starkey (editor)Paperback

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Description

We must unashamedly admit that a large part of the motivation for editing Genomics Protocols was selfish. The possibility of assembling in a single volume a unique and comprehensive collection of complete protocols, relevant to our work and the work of our colleagues, was too good an opportunity to miss. We are pleased to report, however, that the outcome is something of use not only to those who are experienced practitioners in the genomics field, but is also valuable to the larger community of researchers who have recognized the potential of genomics research and may themselves be beginning to explore the technologies involved. Some of the techniques described in Genomics Protocols are clearly not restricted to the genomics field; indeed, a prerequisite for many procedures in this discipline is that they require an extremely high throughput, beyond the scope of the average investigator. However, what we have endeavored here to achieve is both to compile a collection of procedures concerned with geno- scale investigations and to incorporate the key components of "bottom-up" and "top-down" approaches to gene finding. The technologies described extend from those traditionally recognized as coming under the genomics umbrella, touch on proteomics (the study of the expressed protein complement of the genome), through to early therapeutic approaches utilizing the potential of genome programs via gene therapy (Chapters 27-30).

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Contents

Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse Paul A. Lyons Genetic Analysis of Complex Traits Stephen P. Bryant and Mathias N. Chiano Sequence-Based Detection of Single Nucleotide Polymorphisms Deborah A. Nickerson, Natali Kolker, Scott L. Taylor, and Mark J. Rieder Genomic Mismatch Scanning for the Mapping of Genetic Traits Farideh Mirzayans and Michael A. Walter Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization Mario A. J. A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer, and Jan P. A. Baak Construction of a Bacterial Artificial Chromosome Library Sangdun Choi and Ung-Jin Kim Contiguation of Bacterial Clones Sean J. Humphray, Susan J. Knaggs, and Ioannis Ragoussis Mapping of Genomic Clones by Fluorescence In Situ Hybridization Margaret A. Leversha Map Integration: From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map Panagiotis Deloukas Construction of Full-Length-Enriched cDNA Libraries: The Oligo-Capping Method Yutaka Suzuki and Sumio Sugano Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping: The Human Gene Map Panagiotis Deloukas Preparation and Screening of High-Density cDNA Arrays with Genomic Clones Gunther Zehetner, Maria Pack, and Katja Schafer Direct Selection of cDNAs by Genomic Clones Daniela Toniolo Exon Trapping: Application of a Large-Insert Multiple-Exon-Trapping System Martin C. Wapenaar and Johan T. Den Dunnen Sequencing Bacterial Artificial Chromosomes David E. Harris and Lee Murphy Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics Yvonne J. K. Edwards and Simon M. Brocklehurst Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning GregElgar Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization Oliver Dorian von Stein Isolation of Differentially Expressed Genes by Representational Difference Analysis Christine Wallrapp and Thomas M. Gress Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display Michael P. Starkey Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos Nicolas Pollet and Christof Niehrs Expression Monitoring Using cDNA Microarrays: A General Protocol Xing Jian Lou, Mark Schena, Frank T. Horrigan, Richard M. Lawn, and Ronald W. Davis Prediction of Protein Structure and Function by Using Bioinformatics Yvonne J. K. Edwards and Amanda Cottage Identification of Novel Genes by Gene Trap Mutagenesis Anne K. Voss and Tim Thomas Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice Ahmed Mansouri Genomic Analysis Utilizing the Yeast Two-Hybrid System Ilya G. Serebriiskii, Garabet G. Toby, Russell L. FInley, Jr., and Erica A. Golemis Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle Terry J. Amiss and Richard Jude Samulski Retroviral-Mediated Gene Transduction Donald S. Anson Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods Ruth Gjerset, Ali Haghighi, Svetlana Lebedeva, and Dan Mercola Ribozyme Gene Therapy Leonidas A. Phylactou

Product Details

  • publication date: 10/06/2001
  • ISBN13: 9780896037083
  • Format: Paperback
  • Number Of Pages: 552
  • ID: 9780896037083
  • weight: 807
  • ISBN10: 0896037088

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