HPLC Made to Measure: A Practical Handbook for Optimization

HPLC Made to Measure: A Practical Handbook for Optimization

By: Stavros Kromidas (editor), Hans-Joachim Kuss (editor)Hardback

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The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness. This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports.

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About Author

Stavros Kromidas worked as Sales Manager for Waters Chromatography following his PhD thesis completed in 1983. From 1989 to 2001 he was Managing Director of Novia GmbH, a consultant company for analytical chemistry and is an independent consultant since then. He has worked in the area of HPLC since 1978 and has given lectures and training courses since 1984. He is author and co-author of several articles and numerous successful books.


Foreword. Preface. List of Contributors. Structure of the Book. 1 Fundamentals of Optimization. 1.1 Principles of the Optimization of HPLC Illustrated by RP-Chromatography (Stavros Kromidas). 1.2 Fast Gradient Separations (Uwe D. Neue, Yung-Fong Cheng, and Ziling Lu). 1.3 pH and Selectivity in RP-Chromatography (Uwe D. Neue, Alberto Mendez, KimVan Tran, and Diane M. Diehl). 1.4 Selecting the Correct pH Value for HPLC (Michael McBrien). 1.5 Optimization of the Evaluation in Chromatography (Hans-Joachim Kuss). 1.6 Calibration Characteristics and Uncertainty - Indicating Starting Points to Optimize Methods (Stefan Schomer). 2 Characteristics of Optimization in Individual HPLC Modes. 2.1 RP-HPLC. 2.2 Optimization in Normal-Phase HPLC (Veronika R. Meyer). 2.3 Optimization of GPC/SEC Separations by Appropriate Selection of the Stationary Phase and Detection Mode (Peter Kilz). 2.4 Gel Filtration/Size-Exclusion Chromatography (SEC) of Biopolymers - Optimization Strategies and Troubleshooting (Milena Quaglia, Egidijus Machtejevas, Tom Hennessy, and Klaus K. Unger). 2.5 Optimization in Affinity Chromatography (Egbert Muller). 2.6 Optimization of Enantiomer Separations in HPLC (Markus Juza). 2.7 Miniaturization. 3 Coupling Techniques. 3.1 Immunochromatographic Techniques (Michael G. Weller). 3.2 Enhanced Characterization and Comprehensive Analyses by Two-Dimensional Chromatography (Peter Kilz). 3.3 LC/MS - Hints and Recommendations on Optimization and Troubleshooting (Friedrich Mandel). 3.4 LC-NMR Coupling (Klaus Albert, Manfred Krucker, Karsten Putzbach, and Marc D. Grynbaum). 4 Computer-Aided Optimization. 4.1 Computer-Facilitated HPLC Method Development Using DryLab Software (Lloyd R. Snyder and Loren Wrisley). 4.2 ChromSword(r) Software for Automated and Computer-Assisted Development of HPLC Methods (Sergey Galushko, Vsevolod Tanchuk, Irina Shishkina, Oleg Pylypchenko, and Wolf-Dieter Beinert). 4.3 Multifactorial Systematic Method Development and Optimization in Reversed-Phase HPLC (Michael Pfeffer). 5 User Reports. 5.1 Nano-LC-MS/MS in Proteomics (Heike Schafer, Christiane Lohaus, Helmut E. Meyer, and Katrin Marcus). 5.2 Verification Methods for Robustness in RP-HPLC (Hans Bilke). 5.3 Separation of Complex Sample Mixtures (Knut Wagner). 5.4 Evaluation of an Integrated Procedure for the Characterization of Chemical Libraries on the Basis of HPLC-UV/MS/CLND (Mario Arangio, Federico R. Sirtori, Katia Marcucci, Giuseppe Razzano, Maristella Colombo, Roberto Biancardi, and Vincenzo Rizzo). Appendix. Subject Index.

Product Details

  • publication date: 20/02/2006
  • ISBN13: 9783527313778
  • Format: Hardback
  • Number Of Pages: 786
  • ID: 9783527313778
  • weight: 1604
  • ISBN10: 352731377X

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