Human Embryonic Stem Cell Protocols (Methods in Molecular Biology 331)

Human Embryonic Stem Cell Protocols (Methods in Molecular Biology 331)

Paperback

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Description

A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biologya series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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Contents

Human Embryonic Stem Cells: Isolation, Maintenance, and Differentiation Kursad Turksen and Tammy-Claire Troy Blastocyst Culture for Deriving Human Embryonic Stem Cells Ariff Bongso Propagation of Human Embryonic Stem Cells on Human Feeder Cells Mark Richards and Ariff Bongso Derivation and Maintenance of Human Embryonic Stem Cells Michal Amit and Joseph Itskovitz-Eldor Isolation and Characterization of Human Embryonic Stem Cells Maisam Mitalipova and Gail Palmarini Routine Culture and Differentiation of Human Embryonic Stem Cells Jim McWhir, Davina Wojtacha, and Alison Thomson Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells Gautam Dravid, Holly Hammond, and Linzhao Cheng Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions Michal Amit and Joseph Itskovitz-Eldor Manipulation of Self-Renewal in Human Embryonic Stem Cells Through a Novel Pharmacological GSK-3 Inhibitor Noboru Sato and Ali H. Brivanlou Derivation of Human Feeders for Prolonged Support of Human Embryonic Stem Cells Chui-Yee Fong and Ariff Bongso Transplantation of Human Embryonic Stem Cells to the Chick Embryo Ronald S. Goldstein Derivation and Characterization of Neuronal Precursors and Dopaminergic Neurons From Human Embryonic Stem Cells In Vitro Melissa Carpenter, Mahendra S. Rao, William J. Freed, and Xianmin Zeng In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells Xue-Jun Li and Su-Chun Zhang Derivation and Characterization of Hematopoietic Cells From Human Embryonic Stem Cells Lisheng Wang, Chantal Cerdan, Pablo Menendez, and Mickie Bhatia Retroviral Transduction of Hematopoietic Progenitors Derived From Human Embryonic Stem Cells Pablo Menendez, Lisheng Wang, Chantal Cerdan, and Mickie Bhatia Genetic Manipulation of Human Embryonic Stem Cells by Transfection Rachel Eiges Designing, Testing, and Validating a Microarray for Stem Cell Characterization Yongquan Luo, Bhaskar Bhattacharya, Amy X. Yang, Raj K. Puri, and Mahendra S. Rao Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells Noboru Sato and Ali H. Brivanlou Massively Parallel Signature Sequencing Daixing Zhou, Mahendra S. Rao, Roger Walker, Irina Khrebtukova, Christian D. Haudenschild, Takumi Miura, Shannon Decola, Eric Vermaas, Keith Moon, and Thomas J. Vasicek Confrontation Cultures of Embryonic Stem Cells With Multicellular Tumor Spheroids to Study Tumor-Induced Angiogenesis Maria Wartenberg, Andreas Finkensieper, Jurgen Hescheler, and Heinrich Sauer A Novel Experimental Platform for Investigating Cancer Growth and Anti-Cancer Therapy in a Human Tissue Microenvironment Derived From Human Embryonic Stem Cells, Maty Tzukerman and Karl L. Skorecki The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells Justin C. St. John, Alexandra Amaral, Emma Bowles, Joao Facucho Oliveira, Rhiannon Lloyd, Mariana Freitas, Heather L. Gray, Christopher S. Navara, Gisela Oliveira, Gerald P. Schatten, Emma Spikings, and Joao Ramalho-Santos Index

Product Details

  • publication date: 28/10/2010
  • ISBN13: 9781617376009
  • Format: Paperback
  • Number Of Pages: 408
  • ID: 9781617376009
  • weight: 584
  • ISBN10: 1617376000

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  • Saver Delivery: Yes
  • 1st Class Delivery: Yes
  • Courier Delivery: Yes
  • Store Delivery: Yes

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