The Springer Lab Manual about immobilized cells is structured in such a way that guidelines were followed and translated to some practical methods. Like for the guidelines for immobilized cells a structure was chosen in which general aspects of immobilization were covered, the kinetics were described and engineering aspects were covered. Besides laboratory methods some simple calculation procedures have been given in this lab manual. The combination of the practical methods with the calculations provide a tool to do research with immobilized cells. The lab manual concludes with some recent, promising and practical cases of immobilized cells as examples of applications with potential.
Characterization of immobilized cells * Description of the support material * Description of the immobilisation procedure * Measurement of density, particle size and shape of support * Mechanical stability of the support * Diffusion coefficients of metabolites * Quantitity of biomass immobilized, determination of biomass concentration * Kinetics of the suspended cells * Diffusion limitation * Micro-electrodes * Biomass gradients * NMR and immobilized cells * Immobilisation at Large Scale by dispersion * Immobilization at large scale by the resonance nozzle technique * External Mass transfer * Liquid fluidization of gel bead particles * Gradients in liquid, gas or solid fractions * Support material stability at the process conditions * Immobilized cells in food technology: Storage stability, sensitivity to contamination and safety * And other contributions * Subject index.
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- ID: 9783540670704
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