Immunoassays: A Practical Approach (Practical Approach Series No.228)

Immunoassays: A Practical Approach (Practical Approach Series No.228)

By: James Gosling (editor)Paperback

More than 4 weeks availability

£85.00 With FREE Saver Delivery


Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.

Create a review


Analysis by specific binding ; Raising antibodies ; Choosing and characterizing antibodies ; Labels and endpoints ; Solid-phase reagents ; Standard and immunogens ; Optimization ; Validation ; Data processing ; Quality assurance

Product Details

  • publication date: 15/06/2000
  • ISBN13: 9780199637102
  • Format: Paperback
  • Number Of Pages: 324
  • ID: 9780199637102
  • weight: 714
  • ISBN10: 0199637105

Delivery Information

  • Saver Delivery: Yes
  • 1st Class Delivery: Yes
  • Courier Delivery: Yes
  • Store Delivery: Yes

Prices are for internet purchases only. Prices and availability in WHSmith Stores may vary significantly