Immunoassays: A Practical Approach (Practical Approach Series No.228)
By: James Gosling (editor)Paperback
More than 4 weeks availability
Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.
Analysis by specific binding ; Raising antibodies ; Choosing and characterizing antibodies ; Labels and endpoints ; Solid-phase reagents ; Standard and immunogens ; Optimization ; Validation ; Data processing ; Quality assurance
Number Of Pages:
- ID: 9780199637102
- Saver Delivery: Yes
- 1st Class Delivery: Yes
- Courier Delivery: Yes
- Store Delivery: Yes
Prices are for internet purchases only. Prices and availability in WHSmith Stores may vary significantly
© Copyright 2013 - 2017 WHSmith and its suppliers.
WHSmith High Street Limited Greenbridge Road, Swindon, Wiltshire, United Kingdom, SN3 3LD, VAT GB238 5548 36