Immunochemical Protocols: 10 (Methods in Molecular Biology v. 10)

Immunochemical Protocols: 10 (Methods in Molecular Biology v. 10)

By: Margaret M. Manson (author)Hardback

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Highlights recent advances in immunochemical techniques and their impact on basic research and clinical medicine, combining the forces of experts in a wide range of subspecialties to cover a wealth of information.

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Production of Polyclonal Antisera. Efficient Elution of Purified Proteins from Polyvinylidene Difluoride Membranes (Immobilon) After Transfer from SDS-PAGE and Their Use as Immunogens. Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen. Synthesis of Peptides for Use as Immunogens. Production and Characterization of Antibodies Against Synthetic Peptides. Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins. Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase. Purification of Immunoglobulin G (IgG). Epitope Mapping. Enzyme-Antienzyme Method for Immunohistochemistry. Double Label Immunohistochemistry on Tissue Sections Using Alkaline Phosphatase and Peroxidase Conjugates. Immunohistochemical Detection of Bromodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic Studies. Avidin-Biotin Technology: Preparaton of Biotinylated Probes. Preparation of Avidin Conjugates. Immunochemical Applications of Avidin-Biotin Technology. Preparation of Gold Probes. Immunogold Probes for Light Microscopy. Immunogold Probes in Electron Microscopy. Electron Microscopic Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species. Quantitative and Qualitative Immunoelectrophoresis: General Comments on Principles, Reagents, Equipment, and Procedures. Rocket Immunoelectrophoresis Technique or Electroimmunodiffusion. Crossed Immunoelectrophoresis. Crossed Immunoaffinoelectrophoresis. Immunodetection of Proteins by Western Blotting. Erasable Western Blots. Colloidal Gold Staining and Immunoprobing on the Same Western Blot. Colloidal Gold Staining and Immunodetection in 2-D Protein Mapping. Fluorescent Protein Staining on Nitrocellulose with Subsequent Immunodetection of Antigen. Competitive ELISA. Twin-Site ELISAs for fos and myc Oncoproteins Using the Ampak System. Preparation of Cytotoxic Antibody-Toxin Conjugates. Immunoaffinity Purification and Quantification of Antibody-Toxin Conjugates. An Immuno-Slot-Blot Assay for Detection and Quantitation of Alkyldeoxyguanosines in DNA. Production of Monoclonal Antibodies for the Detection of Chemically Modified DNA. Sensitive Competitive Enzyme-Linked Immunoassay for Quantitation of Modified Bases in DNA. An Immunochemical Assay for Detecting Transition of B-DNA to Z-DNA. Cell Sorting Using Immunomagnetic Beads. Cell Preparation for Flow Cytometry. Preparation of Rat Lung Cells for Flow Cytometry. The Isolation of Rat Hepatocytes for Flow Cytometry. Flow Cytometric Analysis of Cells Using an Antibody to a Surface Antigen. Multiple Immunofluorescence Analysis of Cells Using Flow Cytometry. Cell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine. Production and Use of Nonradioactive Hybridization Probes. Cellular Human and Viral DNA Detection by Nonisotopic In Situ Hybridization. Chromosomal Mapping of Genes by Nonisotopic In Situ Hybridization. Nonisotopic In Situ Hybridization: Immunocytochemical Detection of Specific Repetitive Sequences on Chromosomes and Interphase Nuclei. Biotinylated Probes in Colony Hybridization. Screening of lgt11 cDNA Libraries Using Monoclonal Antibodies. Expression of Foreign Genes in Mammalian Cells Using an Antibody Fusion System. Index.

Product Details

  • publication date: 10/04/1992
  • ISBN13: 9780896032040
  • Format: Hardback, Paperback
  • Number Of Pages: 480
  • ID: 9780896032040
  • weight: 875
  • ISBN10: 0896032043

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