Immunological Techniques in Microbiology (Society for Applied Bacteriology Technical Series 24)
By: J. M. Grange (editor), K. R. Fox (editor), N. L. Morgan (editor)Hardback
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In recent years there have been many technical developments in immunology that have potential or actual applications in many other branches of the life sciences. These developments include a number of ultrasensitive systems for the detection and assay of a wide range of biological materials, including micro-organisms and their products, as well as techniques for isolating and purifying micro-organisms and other antigenic materials. Two developments in particular have greatly extended the scope and applicability of immunological methods, namely the production of monoclonal antibodies and the intorudction of enzyme linked immunoassay as a simple, safe and economical alternative to radioimmunoassay. This book is based on the Society for Applied Bacteriology Autumn Demonstration Meeting held at the South Bank Polytechnic, London on October 15th, 1986.
Production of monoclonal antibodies; Purification and analysis of monoclonal antibodies from mouse ascites fluid by high performance ion-exchange and size-exclusion chromatography; Measurement of specific antibodies in the four subclasses of IgG by ELISA; ELISA for the detection of total serum IgE: speed and sensitivity; Magnetic enzyme immunoassay (MEIA) for staphyloccocal enterotoxin B; The potential use of monoclonal antibodies in the diagnosis of tuberculosis; The avidin-biotin technique in immunocytochemical staining; Immunogold labelling of fungal enzymes; Immunofluorescent techniques for legionnaries' disease; Immunofluorescence techniques in plant pathology; Counter-current immunoelectrophoresis for the rapid detection of bacterial polysaccharide antigen in body fluids
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- ID: 9780632019083
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