In Situ Molecular Pathology and Co-Expression Analyses
By: Gerard J. Nuovo (editor)Hardback
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In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical investigators must have a solid understanding of the basics of in situ-based tests, protocols, and regimens for troubleshooting. Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this book's clear presentation of protocols and theoretical framework. It includes over 200 easy-to-follow experimental protocols. It features chapter-ending summaries of "Key Points to Remember" to bring beginners up to speed with any seasoned veteran in the field.
It offers two chapters written by industry leaders in the fields of in situ hybridization, immunohistochemistry, and computer software for co-expression analyses.
Gerard Nuovo received his MD from the University of Vermont College of Medicine in 1983. He did his training in Anatomic Pathology at Columbia Prebyterian College of Physicians and Surgeons in NYC. He then did a two year fellowship at Columbia Presbyterian in Molecular Virology and Gynecologic Pathology under the mentorship of Drs. Saul Silverstein, Chris Crum, and Ralph Richart. Dr. Nuovo's career has been dedicated to bridging the areas of surgical pathology and molecular pathology. He has published over 310 peer review papers in the field and written several textbooks in both Anatomic Pathology and Molecular Pathology. This textbook fulfills a strong ambition of Dr. Nuovo; to empower the next generation of researchers and diagnosticians to include in situ molecular pathology in their work by using easy-to-follow and easy-to-understand protocols for in situ hybridization, immunohistochemistry, and co-expression analyses.
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- ID: 9780124159440
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