This fully updated new edition of a successful and popular practical guide is an indispensable account of modern in-vitro fertilization practice. Initial chapters cover theoretical aspects of gametogenesis and embryo development at the cellular and molecular level, while the latter half of the book describes the requisites for a successful IVF laboratory and the basic technologies in ART. Advanced techniques, including pre-implantation genetic diagnosis, vitrification and stem-cell technology, are comprehensively covered, providing up-to-date analyses of these groundbreaking technologies. This edition includes: * New practical techniques, including preservation of fertility for cancer patients, stem-cell biology/technology, vitrification and in-vitro maturation * A 'refresher' study review of fundamental principles of cell and molecular biology * The latest information available from animal and human research in reproductive biology Packed with a wealth of practical and scientific detail, this is a must for all IVF practitioners.
Kay Elder is Senior Research Scientist, Bourn Hall Clinic, Bourn, Cambridge, UK. Brian Dale is Director of the Centre for Assisted Fertilization, Clinica Villa del Sole, Naples, Italy.
Preface; 1. Review of cell and molecular biology; 2. Endocrine control of reproduction: Controlled Ovarian Hyperstimulation for ART; 3. Gametes and gametogenesis; 4. Sperm-oocyte interaction; 5. First stages of development; 6. Implantation and early stages of fetal development; 7. Stem cell biology; 8. The clinical in vitro fertilization laboratory; 9. Quality management in the IVF laboratory; 10. Sperm and ART; 11. Oocyte retrieval and embryo culture; 12. Cryopreservation of gametes and embryos; 13. Micromanipulation techniques; 14. Preimplantation genetic diagnosis Joyce Harper; 15. Epigenetics and assisted reproduction John Huntriss; Index.