As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.
Part 1: Isolation and Propagation of Cell Populations Isolation and Propagation of Glomerular Mesangial Cells Paolo Mene and Antonella Stoppacciaro Isolation and Primary Culture of Human Proximal Tubule Cells David A. Vesey, Weier Qi, Xinming Chen, Carol A. Pollock and David W. Johnson Propagation and Culture of Renal Fibroblasts Lauren Grimwood and Rosemary Masterson Part 2: Animal Models Small Animal Models of Kidney Disease: A Review Tim D. Hewitson, Takahiko Ono and Gavin J. Becker Part 3: Techniques and Applications Measurement of Glomerular Filtration Rate in Conscious Mice Zhonghua Qi and Matthew D. Breyer Laser Capture Microdissection of Kidney Tissue Robert P. Woroniecki and Erwin P. Bottinger Quantitative Gene Expression Analysis in Kidney Tissues Chris Tikellis, Philip Koh, Wendy Burns and Phillip Kantharidis In vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy A. Richard Kitching, Michael P. Kuligowski and Michael J. Hickey Using In situ Hybridisation to Localize Renal Gene Expression in Tissue Sections Ian A. Darby, Alexis Desmouliere & Tim D. Hewitson Immuno and Lectin Histochemistry for Renal Light Microscopy Tim D. Hewitson and Lauren Grimwood Immuno and Lectin Histochemistry for Renal Electron Microscopy Mitsuru Nakajima Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia Christian Rosenberger, Seymour Rosen, Alexander Paliege and Samuel N. Heyman Identification of Apoptosis in Kidney Tissue Sections Glenda Gobe Cell Populated Floating Collagen Lattices: An in vitro Model of Parenchymal Contraction Kristen J. Kelynack Mechanical Stretch-induced Signal Transduction in Cultured Mesangial Cells Joan Krepinsky Determination of Collagen Content, Concentration and Sub-types in Kidney Tissue Chrishan S. Samuel SELDI-TOF Mass Spectrometry-based Protein Profiling of Kidney Tissue Eleni Giannakis, Chrishan S. Samuel, Wee-Ming Boon, Mary Macris, Tim D. Hewitson and John D. Wade In vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli Yoshitsugu Takabatake, Yoshitaka Isaka and Enyu Imai
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