Containing 57 thoroughly class-tested and easily customizable exercises, Laboratory Experiments in Microbiology, Tenth Edition, provides engaging labs with instruction on performing basic microbiology techniques and applications for undergraduate students in diverse areas, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The perfect companion to Tortora/Funke/Case's Microbiology: An Introduction or any introductory microbiology text, the Tenth Edition features an updated art program and a full-color design, integrating valuable micrographs throughout each exercise. Additionally, many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style to better visually engage students. Laboratory Reports for each exercise have been enhanced with new Clinical Applications questions, as well as questions relating to Hypotheses or Expected Results. Experiments have been refined throughout the manual and the Tenth Edition includes an extensively revised exercise on transformation in bacteria using pGLO to introduce students to this important technique.
Introduction I. MICROSCOPY 1. Use and Care of the Microscope 2. Examination of Living Microorganisms II. Handling Bacteria 3. Microbes in the Environment 4. Transfer of Bacteria: Aseptic Techniques III. STAINING METHODS 5. Preparation of Smears and Simple Staining 6. Negative Staining 7. Gram Staining 8. Acid-Fast Staining 9. Structural Stains (Endospore, Capsule, Flagella) 10. Morphologic Unknown IV. CULTIVATION OF BACTERIA 11. Isolation of Bacteria by Dilution Techniques 12. Special Media for Isolating Bacteria V. MICROBIAL METABOLISM 13. Carbohydrate Catabolism 14. Fermentation 15. Protein Catabolism, Part 1 16. Protein Catabolism, Part 2 17. Respiration 18. Unknown Identification and Bergey's Manual VI. MICROBIAL GROWTH 19. Oxygen and the Growth of Bacteria 20. Determination of a Bacterial Growth Curve: The Role of Temperature 21. Biofilms VII. CONTROL OF MICROBIAL GROWTH 22. Physical Methods of Control: Heat 23. Physical Methods of Control: Ultraviolet Radiation 24. Chemical Methods of Control: Disinfectants and Antiseptics 25. Chemical Methods of Control: Antimicrobial Drugs 26. Effectiveness of Hand Scrubbing VIII. MICROBIAL GENETICS 27. Regulation of Gene Expression 28. Isolation of Bacterial Mutants 29. Transformation of Bacteria 30. DNA Fingerprinting 31. Genetic Engineering 32. Ames Test for Detecting Possible Chemical Carcinogens IX. THE MICROBIAL WORLD 33. Fungi: Yeasts and Molds 34. Phototrophs: Algae and Cyanobacteria 35. Protozoa 36. Parasitic Helminths X. VIRUSES 37. Isolation and Titration of Bacteriophages 38. Plant Viruses XI. INTERACTION OF MICROBE AND HOST 39. Epidemiology 40. Koch's Postulates XII. IMMUNOLOGY 41. Innate Immunology 42. Agglutination Reactions: Slide Agglutination 43. Agglutination Reactions: Microtiter Agglutination 44. ELISA Technique XIII. MICROORGANISMS AND DISEASE 45. Bacteria of the Skin 46. Bacteria of the Respiratory Tract 47. Bacteria of the Mouth 48. Bacteria of the Gastrointestinal Tract 49. Bacteria of the Genitourinary Tract 50. Identification of an Unknown from a Clinical Sample 51. Rapid Identification Methods XIV. MICROBIOLOGY AND THE ENVIRONMENT 52. Microbes in Water: Multiple-Tube Technique 53. Microbes in Water: Membrane Filter Technique 54. Microbes in Food: Contamination 55. Microbes Used in the Production of Foods 56. Microbes in Soil: The Nitrogen and Sulfur Cycles 57. Microbes in Soil: Bioremediaton APPENDICES A. Pipetting B. Dilution Techniques and Calculations C. Use of the Spectrophotometer D. Graphing E. Use of the Dissecting Membrane F. Use of the Membrane Filter G. Electrophoresis H. Keys to Bacteria Index