Methods in Tau Cell Biology, Volume 141, the latest release in the Methods in Cell Biology series, looks at methods involved in tau cell biology. Edited by leaders in the field, this volume provides proven, state-of-art techniques and relevant historical background and theory that aids researchers with tactics for efficient design and effective implementation of experimental methodologies. Topics of note in this updated volume include sections on Recombinant tau expression and purification, In vitro MT dynamics and MT ends, Methods related to investigating tau structure and MT bundling, Neurite outgrowth and retraction, and Methods related to studying tau fragmentation.
Dr. Feinstein earned his undergraduate degree in Biochemistry at the University of California, Berkeley and his Ph.D. from the Department of Biochemistry and Biophysics at the University of California School of Medicine, San Francisco. Subsequently, he was a postdoctoral fellow in the Department of Neurobiology at the Stanford University School of Medicine until 1986, at which time he joined the faculty at UCSB. He has served as a grant reviewer for the National Institutes of Health, the National Science Foundation and the Cancer Research Coordinating Committee of California. In 2011, he was appointed a National Academies Education Fellow in the Life Sciences by the National Academies of Science and previously has been awarded a UCSB Distinguished Teaching Award. Dr. Feinstein is presently Co-Director of the Neuroscience Research Institute. Dr. Lapointe has earned her undergraduate degree in Biology & Psychology at the University of Minnesota-Morris, and her Ph.D. in Neuroscience from Northwestern Univeristy. Subsequently, she was a postdoctoral fellow in Univeristy of California, Santa Barbara until 2013, and has continued to serve UCSB as an associate specialist. She is now an assistant project scientist at UCSB, and has participated in a number of research projects and publications, which her research is mainly focused on Tau proteins.
1. Recombinant tau expression and purification Stuart Feinstein 2. In vitro MT dynamics and MT ends Nichole LaPointe 3. Methods related to investigating tau structure and MT bundling Cyrus R. Safinya 4. Neurite outgrowth and retraction Brett Cook 5. Methods related to studying tau fragmentation Adrianna Ferrerira 6. Isoform-specific differences in tau in vitro aggregation Truman C. Gamblin 7. Cosedimentation assays of microtubule binding Holly Goodson 8. Methods to study tau aggregation Songi Han 9. Methods to study tau phosphorylation Diane Hanger 10. Structure of tau oligomers Nicholas M. Kanaan 11. Use of mouse models for studies of pathological tau action Virginia Lee and Sneha Narasimhan 12. Application of FRET and FCS to study tau structure-function Elizabeth Rhoades 13. Use of the fly system to study tau action Michelle Steinhilb 14. Single molecule assays with motor proteins Christopher Berger 15. Tau clearance mechanisms in primary neurons with a focus on autophagy Gail Johnson 16. Structural studies of tau Eckhard Mandelkow 17. TIRF assays of tau effects on MT and actin cytoskeletons in vitro Isabelle Arnal 18. TIRF assays of tau interactions with EB1 Isabelle Arnal 19. A novel approach to address tau biology, pathology, and propagation in vivo in mice using Adeno associated viruses Susanne Wegmann