Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.
Anna Porwit MD, Ph.D. is Professor at the Department of Clinical Sciences, Division Oncology and Pathology, Lunds Universitet, Sweden. She is a co-author of approximately 200 original and review papers, and some chapters in the WHO2008 and 2016 classification of hematopoietic malignancies. Marie Christine Bene, PharmSciD-Ph.D., has been a professor of Immunology at the Faculty of Medicine of Nancy, France, between 1993 and 2012 and is now Professor of Hematology and the head of the Hematology Biology Department in the Universite de Nantes, France. She has been co-author with Anna Porwit of several publications as well as chapters of the WHO2008 and 2016 classification of hematopoietic malignancies.
List of contributors; Preface; 1. Flow cytometry in clinical hematopathology: basic principles and data analysis of multiparameter data sets Francis Lacombe and Marie C. Bene; 2. Antigens Marie-Christine Bene and Anna Porwit; 3. Flow cytometry of normal blood, bone marrow and lymphatic tissue Anna Porwit and Marie-Christine Bene; 4. Reactive conditions and other diseases where flow cytometric findings may mimic hematological malignancies Wolfgang Kern, Marie-Christine Bene and Anna Porwit; 5. Examples of immunophenotypic features in various categories of acute leukaemia Marie-Christine Bene and Anna Porwit; 6. Acute lymphoid leukaemias and minimal residual disease in all Giuseppe Basso, Barbara Buldini and Andrea Zangrando; 7. Immunophenotyping of mature B-cell lymphomas Olof Axler and Anna Porwit; 8. Plasma cell myeloma and related disorders Ruth M. de Tute, Andrew C. Rawstron and Roger G. Owen; 9. Mature T-cell Neoplasms and NK-cell malignancies Anne Tierens; 10. Flow cytometric diagnosis of hodgkin lymphoma in lymph nodes Lori Soma, Brent L. Wood and Jonathan R. Fromm; 11. Minimal residual disease in acute myeloid leukaemia Gerrit J. Schuurhuis, Angele Kelder, Gert J. Ossenkoppele, Jacqueline Cloos and Wendelien Zeijlemaker; 12. Ambiguous lineage and mixed phenotype acute leukemia Anna Porwit and Marie-Christine Bene; 13. Flow cytometry in myelodysplastic syndromes T. M. Westers and A. A. van de Loosdrecht; 14. Future applications of flow cytometry and related techniques Marie Christine Bene and Francis Lacombe; Index.