Bioanalytical applications in nanopores are an exciting field of research which have seen rapid development over the last ten plus years. This book focuses uniquely on this research area providing an insight from the world leading experts in the area. Based on the proceedings of the 1st International Conference on Nanopores for Bioanalytical Applications (Lanzarote, 2012), it will provide a snapshot of this exciting field for the nanopores community. It describes the latest experimental and fabrication protocols necessary to carry out nanopore-based experiments and covers a wide variety of topics. These include: biological pores, solid state pores, hybrid structures, fundamental aspects, work on the underlying (bio)physics and applications in biosensing and DNA sequencing. The multidisciplinary approach to this topic makes the book suitable for readers in both academia and industry.
Joshua Edel is Senior Lecturer in Micro and Nanotechnology at Imperial College London. His research areas include nanofluidic devices for analytical and bioanalytical application, single molecule biophysics, ultra-high sensitivity optical detection and microdroplets and microfluidics. Tim Albrecht is Senior Lecturer in the Department of Chemistry at Imperial College London. He focuses his research on electrochemical processes on the nanoscale whilst also carrying our teaching duties and student supervisions.
Ionic Current Detection of DNA Origami Nanostructures with Nanocapillaries; On the Development of New Methods for Ion Channel Structure-Function Measurement; Improved Algorithms for Nanopore Signal Processing; A Variable Cross-Section Pore for Screening Cells for Specific Markers; Salt And Voltage Dependence of the Conductance Blockade Induced by Translocation of DNA And RECA Filaments Through Solid-State Nanopores; Biochemical Sensing with Chemically Modified Synthetic Ion Channels; Label-Free Screening of Niche-to-niche Variation in Satellite Stem Cells Using Functionalized Pores; Combining Fluorescence Imaging and Ionic Current Detection in Nanocapillaries; An Introduction to a New Ion Beam Nanopatterning Instrument and It's Application for Automatic Wafer Scale Nanopore Device Production; pH-Reversed Ionic Current Rectification Displayed by Conically Shaped Nanopore without any Modification; Modelling Solid-State Nanopores with a Combination of the Poisson-Nernst-Planck Equations and Brownian Dynamics; Biomimetic Nanopores with Amphoteric Amino Acid Groups. Effects of a pH Gradient on the Ionic Conductance and Selectivity; Forces on DNA in Confinement as Measured by Optical Tweezers; Potential Dependence of DNA Translocation; Towards Simultaneous Force and Resistive Pulse Sensing in Protein Nanopores Using Optical Tweezers; Stimuli-Triggered Permeation of Ionic Analytes Through Nanopores Functionalised with Responsive Molecules; Fabrication of Nanochannel Arrays for the Selective Transport of Ionic Species; Glycan Analysis Using a Solid State Nanopore; Transport Properties of Nano-Porous Track-Etched Membranes in Electrolyte Solutions; Single-Molecule DNA Translocation through Si3N4- and Graphene Solid-State Nanopores; Parallel, High-Resolution Nanopore Analysis on a Chip-based Lipid Membrane Micorarray; Subject Index