Phenotyping of Human iPSC-derived Neurons: Patient-Driven Research
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Phenotyping of Human iPSC-derived Neurons: Patient-Driven Research

Phenotyping of Human iPSC-derived Neurons: Patient-Driven Research

By
Elizabeth D. Buttermore (Contributor)  

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Phenotyping of Human iPSC-derived Neurons: Patient-Driven Research examines the steps in a preclinical pipeline that utilizes iPSC-derived neuronal technology to better understand neurological disorders and identify novel therapeutics, also providing considerations and best practices. By presenting example projects that identify phenotypes and mechanisms relevant to autism spectrum disorder and epilepsy, this book allows readers to understand what considerations are important to assess at the start of project design. Sections address reproducibility issues and advances in technology at each stage of the pipeline and provide suggestions for improvement. From patient sample collection and proper controls to neuronal differentiation, phenotyping, screening, and considerations for moving to the clinic, these detailed descriptions of each stage of the pipeline will help everyone, regardless of stage in the pipeline. In recent years, drug discovery in the neurosciences has struggled to identify novel therapeutics for patients with varying indications, including epilepsy, chronic pain, and psychosis. Current treatment options for such patients are decades old and offer little relief with many side effects. One explanation for this lull in novel therapeutics is a lack of novel target identification for neurological disorders (and target identification requires exemplar preclinical data). To improve on the preclinical work that often relies on rodent modeling, the field has begun utilizing patient-derived induced pluripotent stem cells (iPSCs) to differentiate neurons in vitro for preclinical characterization of neurological disease and target identification. Approx. 100 illustrations (100 in full color); Illustrations, unspecified
Dr. Elizabeth Buttermore is currently the Director of Translational In Vitro Models in the Rosamund Stone Zander Translational Neuroscience Center at Boston Children's Hospital (BCH) where she leads the Human Neuron Core and Neurological Repository Core. Elizabeth has 10 years of experience in phenotypic assay development using high content imaging and multielectrode array approaches. One of her goals at BCH is to help standardize the way the field obtains and interprets phenotypic data and to help researchers across academia and industry move their research forward. She completed her postdoctoral work in Clifford Woolf's lab at BCH where she developed protocols for differentiating nociceptive neurons from human fibroblasts and iPSCs and used them to develop models for neuropathy and neuropathic pain. Prior to coming to BCH, Elizabeth completed her PhD in Neurobiology in 2012 at the University of North Carolina, in the lab of Manzoor Bhat, where she studied the organization and maintenance of molecular domains in myelinated axons.
  • Contributor: Elizabeth D. Buttermore
  • Imprint: Academic Press Inc
  • ISBN13: 9780128222775
  • Number of Pages: 372
  • Packaged Dimensions: 152x229mm
  • Packaged Weight: 750
  • Format: Paperback
  • Publisher: Elsevier Science Publishing Co Inc
  • Release Date: 2022-09-15
  • Binding: Paperback / softback
  • Biography: Dr. Elizabeth Buttermore is currently the Director of Translational In Vitro Models in the Rosamund Stone Zander Translational Neuroscience Center at Boston Children's Hospital (BCH) where she leads the Human Neuron Core and Neurological Repository Core. Elizabeth has 10 years of experience in phenotypic assay development using high content imaging and multielectrode array approaches. One of her goals at BCH is to help standardize the way the field obtains and interprets phenotypic data and to help researchers across academia and industry move their research forward. She completed her postdoctoral work in Clifford Woolf's lab at BCH where she developed protocols for differentiating nociceptive neurons from human fibroblasts and iPSCs and used them to develop models for neuropathy and neuropathic pain. Prior to coming to BCH, Elizabeth completed her PhD in Neurobiology in 2012 at the University of North Carolina, in the lab of Manzoor Bhat, where she studied the organization and maintenance of molecular domains in myelinated axons.

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