Given the immense progress achieved in elucidating protein-protein complex structures and in the field of protein interaction modeling, there is great demand for a book that gives interested researchers/students a comprehensive overview of the field. This book does just that. It focuses on what can be learned about protein-protein interactions from the analysis of protein-protein complex structures and interfaces. What are the driving forces for protein-protein association? How can we extract the mechanism of specific recognition from studying protein-protein interfaces? How can this knowledge be used to predict and design protein-protein interactions (interaction regions and complex structures)? What methods are currently employed to design protein-protein interactions, and how can we influence protein-protein interactions by mutagenesis and small-molecule drugs or peptide mimetics?The book consists of about 15 review chapters, written by experts, on the characterization of protein-protein interfaces, structure determination of protein complexes (by NMR and X-ray), theory of protein-protein binding, dynamics of protein interfaces, bioinformatics methods to predict interaction regions, and prediction of protein-protein complex structures (docking and homology modeling of complexes, etc.) and design of protein-protein interactions. It serves as a bridge between studying/analyzing protein-protein complex structures (interfaces), predicting interactions, and influencing/designing interactions.
Introduction to Protein-Protein Interactions: Protein-Protein Complex Determination by X-ray Crystallography and Interface Analysis of Known Structures (J Janin); Protein-Protein Complex Determination and Analysis of Interactions Using NMR Spectroscopy (J Werner); Perspective on Protein-Protein Interactions in Macromolecular Assemblies (R Bahadur); Energetics of Protein-Protein Interactions (I Jelezarov); Kinetics of Complex Formation: Theory and Experiment (R Wade); Prediction of Putative Protein Interaction Regions and Interaction Geometries: Prediction of Protein Interaction Regions Based on Evolutionary Information (O Lichtarge); Prediction of Interaction Regions and Scoring Optimization (M Zacharias); Protein-Protein Docking Methods: A General Overview (C Prevost); Refinement of Docking Solutions (C Camacho); High-Resolution Docking Including Conformational Flexibility (O Furman); Experimental Constraint-Driven Docking (A Bonvin); Design and Targeting of Protein-Protein Interactions by Drugs and Protein Mimetics: Protein-Protein Interaction Modules of Medical Importance (H Sticht); Prediction/Calculation of Protein-Protein Binding Affinities and Mutation Effects (Fiorucci et al.); Design of Protein Mimetics That Interfere with Protein-Protein Interactions (K Mayo); Identifying Molecules to Inhibit Protein-Protein Interactions (T Berg); Combining Bioinformatics and Experiment to Design Inhibitors of Protein-Protein Interactions (G Colombo).