Separating Cells: The basics provides user-friendly and practical guidance to the techniques most commonly used to separate cells. The book offers a concise overview of the fundamental principles and explains the 'what, how and why'. This title will be of considerable interest to newcomers to these techniques.
PREPARATION OF CELL SUSPENSIONS. Diversity of cells. Why separate living cells?. Preparation of suspensions of single cells. Viability measurements. Separation of viable and nonviable cells. Characterization of cells. FRACTIONATION OF CELLS BY SEDIMENTATION METHODS. Introduction. Separation media. Iso-osmotic gradients. Choice of separation method. Separation of cells on the basis of size. Separation of cells on the basis of their density. Concluding remarks. CENTRIFUGAL ELUTRIATION. Introduction. Principles. Equipment. Conditions affecting separation. Applications of centrifugal elutriation. Advantages and disadvantages FREE-FLOW ELECTROPHORESIS. Introduction. Main types of FFE equipment. Buffers. Preparation of cell samples for FFE. FFE of cell samples. Factors affecting FFE. Applications of FFE. AFFINITY SEPARATION. Introduction. Separation by nonmagnetic techniques. Separation by magnetic techniques. FLOW CYTOMETRY. Introduction. The flow cytometer-instrumentation. Types of flow sorting. Factors affecting cell sorting. Separating-purity, yield and sterility. Applications. List of manufacturers and Suppliers. Index.