This first modern book on the topic gives a broad overview on current technology and application areas for single cell approaches in life sciences. Identification and imaging of single cells which is the most widely applied single cell technology, is described in the opening section, including fluorescence based methods, electron tomography and atomic force techniques. The core section of the book covers a wide range of technologies for the handling, manipulation and constituent analysis of individual cells. The final section is dedicated to case studies and specific applications, with examples ranging from cell biology and genetics to molecular medicine. With its wide range to technologies and applications, this book is the perfect companion for all scientists entering this growing field.
Dario Anselmetti holds the chair in Experimental Biophysics and Applied Nanosciences at Bielefeld University (Germany). He obtained his academic degrees in Physics, Mathematics and Astronomy from Basel University (Switzerland), before becoming a post-doc at the IBM Research Laboratories. Later on, he worked for six years in the research department of Ciba-Geigy (now Novartis) as a project leader in nanobiophysics. After co-managing the Solvias spin-off company in Basel, he returned to academia, taking up his current position in 2000. Dr. Anselmetti has authored over 100 scientific publications and is a member of the Academy of Sciences of the state of Nordrhein-Westfalen.
SINGLE CELL ANALYSIS: IMAGING Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force Cellular Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells SINGLE CELL ANALYSIS: TECHNOLOGY Single Cell Proteomics Protein Analysis of Single Cells in Microfluidic Format Single Cell Mass Spectrometry Single-Cell Analysis for Quantitative Systems Biology Optical Stretcher for Single Cells SINGLE CELL ANALYSIS: APPLICATIONS Single Cell Immunology Molecular Characterization of Rare Single Tumor Cells Single Cell Heterogeneity Genome and Transcriptome Analysis of Single Tumour Cells