Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods: Volume 581 (Methods in Enzymology)

Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods: Volume 581 (Methods in Enzymology)


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Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion.

About Author

Associate Professor of Biochemistry, Carver College of Medicine, University of Iowa, USA Associate Professor of Physics and Biophysics, Department of Physics, University of Illinois at Urbana-Champaign, USA


Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases T.L. Pavankumar, J.C. Exell and S.C. Kowalczykowski Single Molecule Imaging with one Color Fluorescence Y. Qiu and S. Myong Measuring Membrane Protein Dimerization Equilibrium in Lipid Bilayers by Single-Molecule Fluorescence Microscopy R. Chadda and J.L. Robertson Fluorescent Labeling of Proteins in Whole Cell Extracts for Single-Molecule Imaging S.R. Hansen, M.L. Rodgers and A.A. Hoskins Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy E.M. Boehm, S. Subramanyam, M. Ghoneim, M.T. Washington and M. Spies How to Measure Separations and Angles Between Intra-Molecular Fluorescent Markers K.I. Mortensen, J. Sung, J.A. Spudich and H. Flyvbjerg Precisely and Accurately Inferring Single-Molecule Rate Constants C.D. Kinz-Thompson, N.A. Bailey, R.L. Gonzalez Quantification of Functional Dynamics of Membrane Proteins Reconstituted in Nanodiscs Membranes by Single Turnover Functional Readout M.E. Moses, P. Hedegard and N.S. Hatzakis Putting Humpty-Dumpty Together: Clustering the Functional Dynamics of Single Biomolecular Machines Such as the Spliceosome C.E. Rohlman, M.R. Blanco and N.G. Walter Single Molecule FRET to Measure Conformational Dynamics of DNA Mimatch Repair Proteins J.W. Gauer, S. LeBlanc, P. Hao, R. Qiu, B. Case, M. Sakato, M.M. Hingorani, D.A. Erie and K.R. Weninger Single Molecule Confocal FRET Microscopy to Dissect Conformational Changes in the Catalytic Cycle of DNA Topoisomerases S. Hartmann, D. Weidlich and D. Klostermeier Probing the Conformational Landscape of DNA Polymerases Using Diffusion-Based Single-Molecule FRET J. Hohlbein and A.N. Kapanidis Methods for Investigating DNA Accessibility with Single Nucleosomes M.D. Gibson, M. Brehove, Y. Luo, J. North and M.G. Poirier Single-Molecule Fluorescence Studies of Fast Protein Folding Z. Wang, L.A. Campos and V. Munoz Single-Molecule Multi-Color FRET Assay for Studying Structural Dynamics of Biomolecules S. Lee, Y. Jang, S.-J. Lee and S. Hohng A Multi-Color Single Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously M. Goetz, P. Wortmann, S. Schmid and T. Hugel Interferometric Scattering Microscopy for the Study of Molecular Motors J. Andrecka, Y. Takagi, K.J. Mickolajczyk, L.G. Lipert, J.R. Sellers, W.O. Hancock, Y.E. Goldman and P. Kukura Enzyme Kinetics in Femtoliter Arrays P. Mogalisetti and D.R. Walt

Product Details

  • ISBN13: 9780128092675
  • Format: Hardback
  • Number Of Pages: 616
  • ID: 9780128092675
  • weight: 1130
  • ISBN10: 012809267X

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