Synthetic DNA: Methods and Protocols (Methods in Molecular Biology 1472 1st ed. 2017)

Synthetic DNA: Methods and Protocols (Methods in Molecular Biology 1472 1st ed. 2017)

By: Randall Hughes (editor)Hardback

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This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology. Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Synthetic DNA: Methods and Protocols aims to help researchers further their research on manipulate DNA sequences.


Part I: Computational Tools for Design and Assembly of Synthetic DNA 1. A Guide to Using STITCHER for Overlapping Assembly PCR Applications Damien M. O'Halloran 2. Synthetic Gene Design Using Codon Optimization On-Line (COOL) Kai Yu, Kok Siong Ang, and Dong-Yup Lee 3. Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering John N. Milligan and Daniel J. Garry Part II: DNA Synthesis, Assembly, and Cloning 4. Simple Cloning by Prolonged Overlap Extension PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli Chao Zhong, Chun You, Ping Wei, and Yi-Heng Percival Zhang 5. SpeedyGenes: Exploiting an Improved Gene Synthesis Method for the Efficient Production of Error-corrected, Synthetic Protein Libraries for Directed Evolution Andrew Currin, Neil Swainston, Philip J. Day, and Douglas Kell 6. BASIC - A Simple and Accurate Modular DNA Assembly Method Marko Storch, Arturo Casini, Ben Mackrow, Tom Ellis, and Geoff Baldwin 7. Enzymatic Synthesis of Single Stranded Clonal Pure Oligonucleotides Cosimo Ducani and Bjorn Hogberg 8. Rapid Assembly of DNA via Ligase Cycling Reaction (LCR) Sunil Chandran 9. PaperClip: A Simple Method for Flexible Multi-part DNA Assembly Maryia Trubitsyna, Chao-Kuo Liu, Alejandro Salinas, Alistair Elfick, and Christopher French 10. The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesis Brian DeDecker 11. Clonetegration Using OSIP Plasmids - One Step DNA Assembly and Site Specific Genomic Integration in Bacteria Lun Cui and Keith Sherwin 12. Generation of DNA Constructs Using the Golden GATEway Cloning Method Stephan Kirchmaier, Katharin Lust, and Jochen Wittbrodt 13. Gene Deletion by Synthesis in Yeast Jinsil Kim, Dong-uk Kim, and Kawang-Lae Hoe 14. Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR) Sunil Chandran and Elaine Shapland 15. Simultaneous Removal of Multiple DNA Segments by Polymerase Chain Reactions Vishnu Krishnamurthy and Kai Zhang 16. Rapid Construction of Recombinant Plasmids by QuickStep-Cloning Pawel Jajesniak and Tuck Seng Wong Part III: Reducing Error in Synthetic DNA 17. Immobilized MutS-mediated Error Removal of Microchip-synthesized DNA Wen Wan, Dongmei Wang, Xiaolian Gao, and Jiong Hong 18. Selection of Error-less Synthetic Genes in Yeast Hisashi Hoshida, Tohru Yarimizu, and Rinji Akada

Product Details

  • ISBN13: 9781493963416
  • Format: Hardback
  • Number Of Pages: 248
  • ID: 9781493963416
  • weight: 917
  • ISBN10: 1493963414
  • edition: 1st ed. 2017

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