John A. Double and Michael J. Thompson have collected a critically important series of novel and essential techniques for studying telomeres and telomerase. These readily reproducible methods provide cutting-edge tools to identify, measure, and analyze telomeres, to determine telomerase expression at the RNA level, to determine telomerase activity, and to detect potential modifiers of this activity. The techniques for assaying telomerase activity range from standard radiological TRAP assays to nonradioactive methods, from non PCR-based methods to techniques using real-time PCR. Telomeres and Telomerase: Methods and Protocols provides the core array of productive techniques needed today to develop telomerase inhibitors or diagnostic/prognostic telomerase markers.
Introduction to Telomeres and Telomerase Michael C. Bibby Detection of Chromosome Ends by Telomere FISH Harry Scherthan Telomere Length Distribution: Digital Image Processing and Statistical Analysis Jean-Patrick Pommier and Laure Sabatier Analysis of Telomerase RNA Gene Expression by In Situ Hybridization W. Nicol Keith, Joseph Sarvesvaran, and Martin Downey Relative Gene Expression in Normal and Tumor Tissue by Quantitative RT-PCR Dennis S. Salonga, Kathleen D. Danenberg, Jean Grem, Ji Min Park, and Peter V. Danenberg Quantitative Detection of Telomerase Components by Real-Time, Online RT-PCR Analysis with the LightCycler Thomas Emrich, Sheng-Yung Chang, Gerlinde Karl, Birgit Panzinger, and Chris Santini Standard TRAP Assay Angelika M. Burger Stretch PCR Assay Jun-ichi Nakayama and Fuyuki Ishikawa Fluorescent Detection of Telomerase Activity Wade K. Aldous, Amber J. Marean, Mary J. DeHart, and Katherine H. Moore Nonradioactive Detection of Telomerase Activity Using a PCR-ELISA-Based Telomeric Repeat Amplification Protocol Thomas Emrich and Gerlinde Karl In Situ TRAP Assay Detection of Telomerase Activity in Cytological Preparations Kazuma Ohyashiki and Junko H. Ohyashiki Biotinylated Primer for Detecting Telomerase Activity Without Amplification Daekyu Sun Whole-Cell and Microcell Fusion for the Identification of Natural Regulators of Telomerase Henriette Gourdeau, Marsha D. Speevak, Lucie Jette, and Mario Chevrette Screening with COMPARE Analysis for Telomerase Inhibitors Imad Naasani, Takao Yamori, and Takashi Tsuruo Telomerase as a Therapeutic Target: Therapeutic Potential of Telomerase Inhibitors John A. Double