Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips.
Dr Sarah E. Maddocks is a Lecturer of Microbiology at Cardiff Metropolitan University, UK. She received a PhD from the University of Reading in 2005 and worked on bacterial virulence before embarking upon investigations into the host-pathogen relationship during infection. Her main research interests involve the study of wound colonisation, bacterial persistence and the shift from colonisation to infection. Rowena Jenkins is a Lecturer in Microbiology at Cardiff Metropolitan University, from which she received her MSc in Biomedical Science and Medical Microbiology and PhD, and a Fellow of the Higher Education Academy. Her main research has focused on the antimicrobial effects of Manuka honey on potentially pathogenic microorganisms in healthcare and wound management. Cellular morphology, physiology, biofilm prevention/disruption, adhesion/invasion, virulence expression and proteomic/genetic expression profiles of organisms such as MRSA and P. aeruginosa have been studied in response to varying treatments with honey. The data generated by these in vitro studies help reveal how antimicrobial agents might be applied in clinical settings. Rowena is also exploring how novel antimicrobial agents can potentiate antibiotics already in use but whose effectiveness is now challenged by antimicrobial resistance.
Chapter 1. Things to Consider Before Preparing to Undertake Your First Polymerase Chain Reaction Chapter 2. Designing and Ordering Your Polymerase Chain Reaction Primers Chapter 3. Analyzing Your Template DNA and/or PCR Product Chapter 4. Quantitative PCR: Things to Consider Chapter 5. Carrying Out Q-PCR Chapter 6. Using PCR for Cloning and Protein Expression Chapter 7. Polymerase Chain Reaction for Knocking Out Genes